Cleavable Linker Incorporation into a Synthetic Dye‐Nanobody‐Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy
Sign Up to like & getrecommendations! Published in 2022 at "Chembiochem"
DOI: 10.1002/cbic.202200395
Abstract: A bright and photostable fluorescent dye with a disulfide (S−S) linker and maleimide group (Rho594‐S2‐mal), as cleavable and reactive sites, was synthesized and conjugated with anti‐GFP nanobodies (NB). The binding of EGFP (FRET donor) with… read more here.
Keywords: sted microscopy; microscopy; linker; dye ... See more keywords
The Use of FRET/FLIM to Study Proteins Interacting with Plant Receptor Kinases.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7063-6_16
Abstract: The investigation of protein interactions in living plant tissue has become of increasing importance in recent years. A high spatial and temporal resolution for the observation of in vivo protein interaction is needed, e.g., in… read more here.
Keywords: fret flim; plant; receptor kinases; plant receptor ... See more keywords
In Vivo Interaction Studies by Measuring Förster Resonance Energy Transfer Through Fluorescence Lifetime Imaging Microscopy (FRET/FLIM).
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7262-3_14
Abstract: Combinations of multiple fluorescent fusion proteins are commonly generated and used for colocalization studies in live cell imaging but also biochemical analysis of protein-protein interactions by co-immunoprecipitation in vitro. Advanced microscopy techniques like Förster resonance… read more here.
Keywords: fret flim; energy transfer; microscopy; interaction ... See more keywords
In cellulo FRET-FLIM and single molecule tracking reveal the supra-molecular organization of the pyoverdine bio-synthetic enzymes in Pseudomonas aeruginosa
Sign Up to like & getrecommendations! Published in 2020 at "Quarterly Reviews of Biophysics"
DOI: 10.1017/s0033583519000155
Abstract: Abstract The bio-synthesis of pyoverdine (PVD) in Pseudomonas aeruginosa involves multiple enzymatic steps including the action of non-ribosomal peptide synthetases (NRPSs). One hallmark of NRPS is their ability to make usage of non-proteinogenic amino-acids synthesized… read more here.
Keywords: single molecule; supra molecular; molecule tracking; bio ... See more keywords
In vivo FRET–FLIM reveals cell-type-specific protein interactions in Arabidopsis roots
Sign Up to like & getrecommendations! Published in 2017 at "Nature"
DOI: 10.1038/nature23317
Abstract: During multicellular development, specification of distinct cell fates is often regulated by the same transcription factors operating differently in distinct cis-regulatory modules, either through different protein complexes, conformational modification of protein complexes, or combinations of… read more here.
Keywords: protein; fret flim; type specific; vivo fret ... See more keywords
In vivo FRET-FLIM reveals ER-specific increases in the ABA level upon environmental stresses.
Sign Up to like & getrecommendations! Published in 2021 at "Plant physiology"
DOI: 10.1093/plphys/kiab165
Abstract: Plant hormone abscisic acid (ABA) is essential for regulating plant growth and various stress responses. ABA-mediated signaling depends on local ABA levels rather than overall cellular ABA concentration. Whereas cellular concentration of ABA can be… read more here.
Keywords: fret flim; specific increases; upon environmental; increases aba ... See more keywords