Super-Resolved FRET Imaging by Confocal Fluorescence-Lifetime Single-Molecule Localization Microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Small methods"
DOI: 10.1002/smtd.202201565
Abstract: Fluorescence Resonance Energy Transfer (FRET)-based approaches are unique tools for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and Fluorescence Lifetime Imaging Microscopy (FLIM) enable the visualization of the spatial distribution of molecular interactions… read more here.
Keywords: super resolved; resolved fret; microscopy; fluorescence lifetime ... See more keywords
Homo-FRET Imaging to Study Protein-Protein Interaction and Complex Formation in Plants.
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DOI: 10.1007/978-1-0716-1791-5_12
Abstract: Protein-protein interactions in living plant cells can be measured by changes in fluorescence anisotropy due to homo-FRET (Förster Resonance Energy Transfer). Here, the energy transfer between identical fluorophores, e.g., enhanced green fluorescent protein (EGFP) fused… read more here.
Keywords: complex formation; protein protein; homo fret; fret imaging ... See more keywords
Automated E‐FRET microscope for dynamical live‐cell FRET imaging
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Microscopy"
DOI: 10.1111/jmi.12783
Abstract: Acceptor‐sensitised 3‐cube fluorescence resonance energy transfer (FRET) imaging (also termed as E‐FRET imaging) is a popular fluorescence intensity‐based FRET quantification method. Here, an automated E‐FRET microscope with user‐friendly interfaces was set up for dynamical online… read more here.
Keywords: live cell; microscope; fret imaging; automated fret ... See more keywords