Joint refinement of FRET measurements using spectroscopic and computational tools.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.01.011
Abstract: The variability of the orientation factor is a long-standing challenge in converting FRET efficiency measurements into donor-acceptor distances. We propose the use of molecular dynamics (MD) simulations to characterize orientation distributions and thus improve the… read more here.
Keywords: orientation; fret; computational tools; donor acceptor ... See more keywords
Hyperspectral Measurements Allow Separation of FRET Signals from Non-Uniform Background Fluorescence
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.2428
Abstract: In recent years Forster resonance energy transfer (FRET) has become a standard imaging approach to gain insight into localized biochemical processes within cells. These processes include changes in protein colocalization, second messenger concentration, and activation… read more here.
Keywords: fluorescence; fret measurements; uniform background; background fluorescence ... See more keywords
Probing the Structural Dynamics of the Activation Gate of KcsA Using Homo-FRET Measurements
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms222111954
Abstract: The allosteric coupling between activation and inactivation processes is a common feature observed in K+ channels. Particularly, in the prokaryotic KcsA channel the K+ conduction process is controlled by the inner gate, which is activated… read more here.
Keywords: homo fret; homo; gate; fret measurements ... See more keywords