Enzymatic synthesis of a fluorogenic reporter substrate and development of a high-throughput assay for fucosyltransferase VIII provide a toolkit to probe and inhibit core fucosylation.
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DOI: 10.1021/acs.biochem.0c00286
Abstract: We report a straight-forward enzymatic synthesis of the 4-methylumbelliferyl glycoside of a complex-type oligosaccharide substrate for core-fucosylation. We demonstrate the use of this synthetic glycoconjugate in a newly developed enzyme assay to probe the activity… read more here.
Keywords: enzymatic synthesis; fucosyltransferase viii; core fucosylation; probe ... See more keywords