Novel Tools for the Functional Expression of Metagenomic DNA.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6691-2_10
Abstract: Functional expression of genes from metagenomic libraries is limited by various factors including inefficient transcription and/or translation of target genes as well as improper folding and assembly of the corresponding proteins caused by the lack… read more here.
Keywords: tools functional; metagenomic dna; novel tools; expression ... See more keywords
Avermectin biosynthesis: stable functional expression of branched chain α-keto acid dehydrogenase complex from Streptomyces avermitilis in Escherichia coli by selectively regulating individual subunit gene expression
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Letters"
DOI: 10.1007/s10529-017-2389-z
Abstract: ObjectiveTo improve the production of short branched-chain acyl-CoAs for avermectin biosynthesis, the functional expression of the branched chain α-keto acid dehydrogenase complex (BKDH) from Streptomyces avermitilis was systematically optimized by selectively regulating individual subunit expression… read more here.
Keywords: individual subunit; bkdh; branched chain; expression ... See more keywords
Challenges of functional expression of complex polyketide biosynthetic gene clusters.
Sign Up to like & getrecommendations! Published in 2021 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2020.12.007
Abstract: Polyketide natural products are valuable sources of bioactive molecules such as nutraceuticals and pharmaceuticals. The tremendous development of the genome sequence database revealed that the majority of the biosynthetic gene clusters (BGCs) are cryptic. Activation… read more here.
Keywords: biosynthetic gene; gene clusters; challenges functional; functional expression ... See more keywords
Feasibility of the functional expression of the human organic anion transporting polypeptide 1B1 (OATP1B1) and its genetic variant 521T/C in the mouse liver
Sign Up to like & getrecommendations! Published in 2017 at "European Journal of Pharmaceutical Sciences"
DOI: 10.1016/j.ejps.2016.09.012
Abstract: &NA; The objective of this study was to examine the feasibility of functional expression of the human organic anion transporting polypeptide 1B1 (hOATP1B1) forms in the liver of the mouse. After the mouse received the… read more here.
Keywords: expression human; feasibility functional; liver; expression ... See more keywords
Functional expression of recombinant hybrid enzymes composed of bacterial and insect's chitinase domains in E. coli.
Sign Up to like & getrecommendations! Published in 2020 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2019.109492
Abstract: To elucidate the functional alteration of the recombinant hybrid chitinases composed of bacterial and insect's domains, we cloned the constitutional domains from chitinase-encoding cDNAs of a bacterial species, Bacillus thuringiensis (BtChi) and a lepidopteran insect… read more here.
Keywords: composed bacterial; recombinant hybrid; expression recombinant; recombinant ... See more keywords
Controlled functional expression of the bacteriocins pediocin PA-1 and bactofencin A in Escherichia coli
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-02868-w
Abstract: The bacteriocins bactofencin A (class IId) and pediocin PA-1 (class IIa) are encoded by operons with a similarly clustered gene organization including a structural peptide, an immunity protein, an ABC transporter and accessory bacteriocin transporter… read more here.
Keywords: expression bacteriocins; pediocin bactofencin; controlled functional; bacteriocins pediocin ... See more keywords
Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-25396-7
Abstract: Transfer of nitrogen fixation ability to plants, especially crops, is a promising approach to mitigate dependence on chemical nitrogen fertilizer and alleviate environmental pollution caused by nitrogen fertilizer run-off. However, the need to transfer a… read more here.
Keywords: expression oxygen; non diazotrophic; nitrogenase; functional expression ... See more keywords
The functional expression of transient receptor potential channels in the mouse endometrium
Sign Up to like & getrecommendations! Published in 2017 at "Human Reproduction"
DOI: 10.1093/humrep/dew344
Abstract: STUDY QUESTION Does mouse endometrial epithelial cells and stromal cells have a similar transient receptor potential (TRP)-channel expression profile and to that found in the human endometrium? SUMMARY ANSWER Mouse endometrial epithelial and stromal cells… read more here.
Keywords: epithelial stromal; trp channels; stromal cells; trp ... See more keywords
Functional Expression of Transient Receptor Potential and Piezo1 Channels in Cultured Interstitial Cells of Human-Bladder Lamina Propria
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Physiology"
DOI: 10.3389/fphys.2021.762847
Abstract: The interstitial cells in bladder lamina propria (LP-ICs) are believed to be involved in sensing/afferent signaling in bladder mucosa. Transient receptor potential (TRP) cation channels act as mechano- or chemo-sensors and may underlie some of… read more here.
Keywords: human bladder; trpa1; bladder; piezo1 ... See more keywords
Sculpting ion channel functional expression with engineered ubiquitin ligases
Sign Up to like & getrecommendations! Published in 2017 at "eLife"
DOI: 10.7554/elife.29744
Abstract: The functional repertoire of surface ion channels is sustained by dynamic processes of trafficking, sorting, and degradation. Dysregulation of these processes underlies diverse ion channelopathies including cardiac arrhythmias and cystic fibrosis. Ubiquitination powerfully regulates multiple… read more here.
Keywords: channel functional; ion channel; expression; ion ... See more keywords
Taxonomic, functional and expression analysis of viral communities associated with marine sponges
Sign Up to like & getrecommendations! Published in 2021 at "PeerJ"
DOI: 10.7717/peerj.10715
Abstract: Viruses play an essential role in shaping the structure and function of ecological communities. Marine sponges have the capacity to filter large volumes of ‘virus-laden’ seawater through their bodies and host dense communities of microbial… read more here.
Keywords: taxonomic functional; viral communities; analysis viral; marine sponges ... See more keywords