A highly sensitive and specific method to evaluate viable fungal burden of Aspergillus fumigatus in mice by RT-qPCR for 18S ribosomal RNA.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2021.106214
Abstract: Potent fungicidal activity is one of the key factors of antifungals to overcome invasive pulmonary aspergillosis (IPA). To date, quantification of Aspergillus DNA in the lungs and galactomannan (GM) in serum or bronchoalveolar lavage fluid… read more here.
Keywords: 18s ribosomal; viable fungal; method; burden ... See more keywords
Murine model of invasive pulmonary Aspergillosis: Follow-up of tissue injury, fungal burden and mortality with distinct elastase production strains.
Sign Up to like & getrecommendations! Published in 2019 at "Journal de mycologie medicale"
DOI: 10.1016/j.mycmed.2018.10.003
Abstract: To study invasive pulmonary Aspergillosis (IPA), we depleted neutrophils in mice using the monoclonal antibody anti-Gr-1/Ly-6G. Immunocompetent and neutropenic mice were infected via intratracheal with conidia of Aspergillus fumigatus clinical isolates, characterized as either higher… read more here.
Keywords: pulmonary aspergillosis; tissue injury; elastase; invasive pulmonary ... See more keywords
2393. Evaluation of Antifungal Treatment in a Neutropenic Mouse Model of Scedosporiosis
Sign Up to like & getrecommendations! Published in 2018 at "Open Forum Infectious Diseases"
DOI: 10.1093/ofid/ofy210.2046
Abstract: Abstract Background Scedosporiosis is a rare fungal infection with high mortality rates. Because clinical trials are hard to conduct, we developed a murine model for evaluating the efficacy of currently used antifungals in treating scedosporiosis.… read more here.
Keywords: treatment; infection; model; scedosporiosis ... See more keywords
The Fungal Cyp51-Specific Inhibitor VT-1598 Demonstrates In Vitro and In Vivo Activity against Candida auris
Sign Up to like & getrecommendations! Published in 2018 at "Antimicrobial Agents and Chemotherapy"
DOI: 10.1128/aac.02233-18
Abstract: Candida auris is an emerging pathogen associated with significant mortality and often multidrug resistance. VT-1598, a tetrazole-based fungal CYP51-specific inhibitor, was evaluated in vitro and in vivo against C. auris. ABSTRACT Candida auris is an… read more here.
Keywords: vitro vivo; specific inhibitor; candida auris; fungal cyp51 ... See more keywords
Unexpected Transcripts in Tn7 orf19.2646 C. albicans Mutant Lead to Low Fungal Burden Phenotype In vivo
Sign Up to like & getrecommendations! Published in 2017 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2017.00873
Abstract: The commensal fungus Candida albicans is the major cause of fungal systemic infection in immuno-compromised patients, with a mortality rate approaching 50% in the case of bloodstream infections. There is therefore a clear need to… read more here.
Keywords: tn7; unexpected transcripts; orf19 2646; phenotype ... See more keywords
Targeting Aspergillus fumigatus Crf Transglycosylases With Neutralizing Antibody Is Relevant but Not Sufficient to Erase Fungal Burden in a Neutropenic Rat Model
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.00600
Abstract: Aspergillus fumigatus is an airborne opportunistic fungal pathogen responsible for severe infections. Among them, invasive pulmonary aspergillosis has become a major concern as mortality rates exceed 50% in immunocompromised hosts. In parallel, allergic bronchopulmonary aspergillosis… read more here.
Keywords: aspergillus fumigatus; neutropenic rat; fungal burden; rat model ... See more keywords