A novel antibody against the furin cleavage site of SARS-CoV-2 spike protein: Effects on proteolytic cleavage and ACE2 binding
Sign Up to like & getrecommendations! Published in 2022 at "Immunology Letters"
DOI: 10.1016/j.imlet.2022.01.002
Abstract: SARS-CoV-2 harbors a unique S1/S2 furin cleavage site within its spike protein, which can be cleaved by furin and other proprotein convertases. Proteolytic activation of SARS-CoV-2 spike protein at the S1/S2 boundary facilitates interaction with… read more here.
Keywords: sars cov; spike protein; cleavage; cleavage site ... See more keywords
The D614G mutation redirects SARS-CoV-2 spike to lysosomes and suppresses deleterious traits of the furin cleavage site insertion mutation
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.ade5085
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) egress occurs by lysosomal exocytosis. We show that the Spike D614G mutation enhances Spike trafficking to lysosomes, drives Spike-mediated reprogramming of lysosomes, and reduces cell surface Spike expression… read more here.
Keywords: d614g mutation; furin cleavage; trafficking lysosomes; mutation ... See more keywords
Acquisition of Furin Cleavage Site and Further SARS-CoV-2 Evolution Change the Mechanisms of Viral Entry, Infection Spread, and Cell Signaling
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Virology"
DOI: 10.1128/jvi.00753-22
Abstract: The results of this study demonstrate that the late lineages of SARS-CoV-2 evolved to more efficient use of the TMPRSS2-mediated entry pathway and gradually lost an ability to employ the cathepsins/endosome-mediated entry. The acquisition of… read more here.
Keywords: cleavage site; cell; entry; furin cleavage ... See more keywords
TMPRSS2 promotes SARS-CoV-2 evasion from NCOA7-mediated restriction.
Sign Up to like & getrecommendations! Published in 2021 at "PLoS pathogens"
DOI: 10.1371/journal.ppat.1009820
Abstract: Interferons play a critical role in regulating host immune responses to SARS-CoV-2, but the interferon (IFN)-stimulated gene (ISG) effectors that inhibit SARS-CoV-2 are not well characterized. The IFN-inducible short isoform of human nuclear receptor coactivator… read more here.
Keywords: restriction; promotes sars; furin cleavage; tmprss2 promotes ... See more keywords
Inclusion of a Furin Cleavage Site Enhances Antitumor Efficacy against Colorectal Cancer Cells of Ribotoxin α-Sarcin- or RNase T1-Based Immunotoxins
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DOI: 10.3390/toxins11100593
Abstract: Immunotoxins are chimeric molecules that combine the specificity of an antibody to recognize and bind tumor antigens with the potency of the enzymatic activity of a toxin, thus, promoting the death of target cells. Among… read more here.
Keywords: ribotoxin sarcin; furin cleavage; inclusion furin; cleavage site ... See more keywords
Propagation of SARS-CoV-2 in Calu-3 Cells to Eliminate Mutations in the Furin Cleavage Site of Spike
Sign Up to like & getrecommendations! Published in 2021 at "Viruses"
DOI: 10.3390/v13122434
Abstract: SARS-CoV-2 pathogenesis, vaccine, and therapeutic studies rely on the use of animals challenged with highly pathogenic virus stocks produced in cell cultures. Ideally, these virus stocks should be genetically and functionally similar to the original… read more here.
Keywords: propagation; sars cov; virus stocks; cleavage site ... See more keywords