Creating Oxidase–Peroxidase Fusion Enzymes as a Toolbox for Cascade Reactions
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DOI: 10.1002/cbic.201700478
Abstract: A set of bifunctional oxidase–peroxidases has been prepared by fusing four distinct oxidases to a peroxidase. Although such fusion enzymes have not been observed in nature, they could be expressed and purified in good yields.… read more here.
Keywords: oxidase peroxidase; cascade reactions; peroxidase; fusion enzymes ... See more keywords
Closing the gap for efficient immobilization of biocatalysts in continuous processes: HaloTag™ fusion enzymes for a continuous enzymatic cascade towards a vicinal chiral diol
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DOI: 10.1039/c7gc03225k
Abstract: Compartmentalization of biocatalysts is an effective tool to integrate biocatalytic steps in continuous (chemo)enzymatic cascades. Therefore, efficient covalent immobilization techniques are of utmost importance, which enable a fast and selective immobilization of the enzyme directly… read more here.
Keywords: diol; immobilization; cascade; enzymatic cascade ... See more keywords
Design of fusion enzymes for biocatalytic applications in aqueous and non-aqueous media
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Bioengineering and Biotechnology"
DOI: 10.3389/fbioe.2022.944226
Abstract: Biocatalytic cascades play a fundamental role in sustainable chemical synthesis. Fusion enzymes are one of the powerful toolboxes to enable the tailored combination of multiple enzymes for efficient cooperative cascades. Especially, this approach offers a… read more here.
Keywords: fusion enzymes; aqueous media; non aqueous; design ... See more keywords