Squid Suckerin‐Spider Silk Fusion Protein Hydrogel for Delivery of Mesenchymal Stem Cell Secretome to Chronic Wounds
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Healthcare Materials"
DOI: 10.1002/adhm.202201900
Abstract: Chronic wounds are non‐healing wounds characterized by a prolonged inflammation phase. Excessive inflammation leads to elevated protease levels and consequently to a decrease in growth factors at wound sites. Stem cell secretome therapy has been… read more here.
Keywords: stem cell; protein hydrogel; fusion protein; chronic wounds ... See more keywords
Targeting the Lysosomal Degradation of Rab22a‐NeoF1 Fusion Protein for Osteosarcoma Lung Metastasis
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202205483
Abstract: Rab22a‐NeoF fusion protein has recently been reported as a promising target for osteosarcoma lung metastasis. However, how this fusion protein is regulated in cells remains unknown. Here, using multiple screenings, it is reported that Rab22a‐NeoF1… read more here.
Keywords: rab22a neof1; osteosarcoma lung; fusion protein; rab22a ... See more keywords
Mechanisms of precipitate formation during the purification of an Fc‐fusion protein
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26746
Abstract: Protein precipitates that arise during bioprocessing can cause manufacturing challenges, but they can also aid in clearance of host‐cell protein (HCP) and DNA impurities. Such precipitates differ from many protein precipitates that have been studied… read more here.
Keywords: protein; precipitate formation; microscopy; fusion protein ... See more keywords
Manipulation of mRNA translation elongation influences the fragmentation of a biotherapeutic Fc‐fusion protein produced in CHO cells
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28230
Abstract: Mammalian cells, particularly Chinese hamster ovary cells, are the dominant system for the production of protein‐based biotherapeutics, however, product degradation, particularly of Fc‐fusion proteins, is sometimes observed that impacts the quality of the protein generated.… read more here.
Keywords: fusion; translation elongation; fusion protein; mrna translation ... See more keywords
A novel multimeric sCD19‐streptavidin fusion protein for functional detection and selective expansion of CD19‐targeted CAR‐T cells
Sign Up to like & getrecommendations! Published in 2022 at "Cancer Medicine"
DOI: 10.1002/cam4.4657
Abstract: CARs are engineered receptors comprising an immunoglobulin single‐chain variable fragment (scFv) that identifies and binds to the target antigen, a transmembrane domain, and an intracellular T‐cell signaling domain. CD19 is a B lineage‐specific transmembrane glycoprotein… read more here.
Keywords: scd19 streptavidin; novel multimeric; fusion protein; streptavidin fusion ... See more keywords
A decoy strategy to activate the immune system
Sign Up to like & getrecommendations! Published in 2021 at "IUBMB Life"
DOI: 10.1002/iub.2548
Abstract: An approach comprising a novel fusion protein and inactivated virus, as a more efficacious vaccine against invading viruses is presented, using SARS‐CoV‐2 as a most prominent example. The fusion protein consists of the Hepatitis B… read more here.
Keywords: fusion protein; immune system; would bind;
Design of a H2O2‐generating P450SPα fusion protein for high yield fatty acid conversion
Sign Up to like & getrecommendations! Published in 2022 at "Protein Science"
DOI: 10.1002/pro.4501
Abstract: Sphingomonas paucimobilis' P450SPα (CYP152B1) is a good candidate as industrial biocatalyst. This enzyme is able to use hydrogen peroxide as unique cofactor to catalyze the fatty acids conversion to α‐hydroxy fatty acids, thus avoiding the… read more here.
Keywords: fusion protein; conversion; h2o2; yield ... See more keywords
In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7253-1_20
Abstract: Extracellular vesicles (EVs) are cell-derived vesicles comprising a lipid bilayer and are found in body fluids, such as blood, sweat, and urine. As EVs, especially exosomes, function as endogenous intercellular delivery tools, their roles in… read more here.
Keywords: protein; extracellular vesicles; vivo; protein comprising ... See more keywords
NKG2D-Fc fusion protein promotes antitumor immunity through the depletion of immunosuppressive cells
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Immunology, Immunotherapy"
DOI: 10.1007/s00262-020-02615-7
Abstract: A major factor impeding the success of numerous therapeutic approaches in cancer is the immunosuppressive nature of the tumor microenvironment (TME). Hence, methods capable of reverting tumor immunosuppression through depletion or reprogramming of myeloid-derived suppressive… read more here.
Keywords: mdscs tregs; antitumor immunity; nkg2d fusion; depletion ... See more keywords
Cloning, Expression and One-Step Purification of a Novel IP-10-(anti-HER2 scFv) Fusion Protein in Escherichia coli
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Peptide Research and Therapeutics"
DOI: 10.1007/s10989-020-10100-z
Abstract: Breast cancer is the most common cancer in women, worldwide. The correlation between breast cancer malignancy and human epidermal growth factor 2 (HER2) expression leads to application of monoclonal antibodies against HER2 in HER2-overexpressing breast… read more here.
Keywords: fusion; anti her2; fusion protein; expression ... See more keywords
Expression of BMP2-Hydrophobin fusion protein in the tobacco plant and molecular dynamic evaluation of its simulated model
Sign Up to like & getrecommendations! Published in 2021 at "Plant Biotechnology Reports"
DOI: 10.1007/s11816-021-00684-3
Abstract: Plants are one of the ideal models for therapeutic protein production, however the recombinant protein purification problems in them must be overcome. Bone Morphogenetic Protein2 (BMP2) is employed for the restoration and construction of bone… read more here.
Keywords: protein; plant; purification; bmp2 ... See more keywords