Development of a temperature‐responsive yeast cell factory using engineered Gal4 as a protein switch
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26544
Abstract: Conflict between cell growth and product accumulation is frequently encountered in biosynthesis of secondary metabolites. Herein, a temperature‐dependent dynamic control strategy was developed by modifying the GAL regulation system to facilitate two‐stage fermentation in yeast.… read more here.
Keywords: gal4; temperature; temperature responsive; yeast ... See more keywords
Refinement of ectopic protein expression through the GAL4/UAS system in Bombyx mori: application to behavioral and developmental studies
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-12102-2
Abstract: Silkmoth, Bombyx mori, is one of the important model insects in which transgenic techniques and the GAL4/UAS system are applicable. However, due to cytotoxicity and low transactivation activity of GAL4, effectiveness of the GAL4/UAS system… read more here.
Keywords: gal4; system; bombyx mori; gal4 uas ... See more keywords
Proteomic mapping of Drosophila transgenic elav.L-GAL4/+ brain as a tool to illuminate neuropathology mechanisms
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-62510-0
Abstract: Drosophila brain has emerged as a powerful model system for the investigation of genes being related to neurological pathologies. To map the proteomic landscape of fly brain, in a high-resolution scale, we herein employed a… read more here.
Keywords: drosophila; gal4; mapping drosophila; elav ... See more keywords
Drosophila Hox genes induce melanized pseudo-tumors when misexpressed in hemocytes
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-81472-5
Abstract: Hox genes are early determinants of cell identity along the anterior–posterior body axis across bilaterians. Several late non-homeotic functions of Hox genes have emerged in a variety of processes involved in organogenesis in several organisms,… read more here.
Keywords: gal4; hox genes; group; pseudo tumors ... See more keywords
Characterizing a gene expression toolkit for eye- and photoreceptor-specific expression in Drosophila
Sign Up to like & getrecommendations! Published in 2021 at "Fly"
DOI: 10.1080/19336934.2021.1915683
Abstract: ABSTRACT Binary expression systems are a powerful tool for tissue- and cell-specific research. Many of the currently available Drosophila eye-specific drivers have not been systematically characterized for their expression level and cell-type specificity in the… read more here.
Keywords: gal4; microscopy; gene expression; toolkit eye ... See more keywords
Using tissue specific P450 expression in Drosophila melanogaster larvae to understand the spatial distribution of pesticide metabolism in feeding assays
Sign Up to like & getrecommendations! Published in 2022 at "Insect Molecular Biology"
DOI: 10.1111/imb.12765
Abstract: Drug metabolizing enzymes such as cytochrome P450s have often been implicated in influencing levels of pesticide toxicology and resistance. Consequently, a variety of different P450 genes and variants have been linked to pesticide metabolism. Substantially… read more here.
Keywords: pesticide metabolism; toxicology; p450; gal4 ... See more keywords
Characterization of a morphogenetic furrow specific Gal4 driver in the developing Drosophila eye
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0196365
Abstract: The ability to express a gene of interest in a spatio-temporal manner using Gal4-UAS system has allowed the use of Drosophila model to study various biological phenomenon. During Drosophila eye development, a synchronous wave of… read more here.
Keywords: gal4; gal4 driver; dpp; gene ... See more keywords
Modeling transcriptional activation changes to Gal4 variants via structure-based computational mutagenesis
Sign Up to like & getrecommendations! Published in 2018 at "PeerJ"
DOI: 10.7717/peerj.4844
Abstract: As a DNA binding transcriptional activator, Gal4 promotes the expression of genes responsible for galactose metabolism. The Gal4 protein from Saccharomyces cerevisiae (baker’s yeast) has become a model for studying eukaryotic transcriptional activation in general… read more here.
Keywords: gal4; mutagenesis; gal4 variants; structure ... See more keywords