Cloning and heterologous expression of the β-galactosidase gene from Bifidobacterium longum RD47 in B. bifidum BGN4.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of microbiology and biotechnology"
DOI: 10.0414/jmb.1905.05068
Abstract: The gene encoding β-galactosidase was cloned from Bifidobacterium longum RD47 with combinations of several bifidobacterial promoters and expressed in B. bifidum BGN4. Among the recombinant bifidobacteria, BGN4+G1 showed the highest β-galactosidase level, for which the… read more here.
Keywords: milk; longum rd47; bgn4; bifidum bgn4 ... See more keywords
A GH42 β‐galactosidase from Bifidobacterium thermophilum: Biochemical characterization and synthesis of galactosyl glycerol by reverse hydrolysis
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.2408
Abstract: In this study, a β‐galactosidase gene (btgal42) was first cloned from Bifidobacterium thermophilum and successfully expressed in Escherichia coli. The molecular weight of the purified BtGal42 was estimated to be 78 kDa by the sodium… read more here.
Keywords: galactosyl glycerol; reverse hydrolysis; glycerol; bifidobacterium thermophilum ... See more keywords
Strong increase of leukocyte apha‐galactosidase A activity in two male patients with Fabry disease following oral chaperone therapy
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Genetics & Genomic Medicine"
DOI: 10.1002/mgg3.894
Abstract: Fabry disease (OMIM 301500) is an X‐linked disorder caused by alpha‐galactosidase A (α‐Gal A) deficiency. The administration of a pharmacologic chaperone (migalastat) in Fabry patients with amenable mutations has been reported to improve or stabilize… read more here.
Keywords: fabry disease; increase; galactosidase; chaperone ... See more keywords
A novel β-galactosidase from Klebsiella oxytoca ZJUH1705 for efficient production of galacto-oligosaccharides from lactose
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10679-9
Abstract: Galacto-oligosaccharides (GOS), which can be produced by enzymatic transgalactosylation of lactose with β-galactosidases, have attracted much attention in recent years because of their prebiotic functions and wide uses in infant formula, infant foods, livestock feed,… read more here.
Keywords: galactosidase; production; activity; oxytoca zjuh1705 ... See more keywords
Comment on ‘‘Elucidating the binding efficacy of β-galactosidase on graphene by docking approach and its potential application in galacto-oligosaccharide production”
Sign Up to like & getrecommendations! Published in 2017 at "Bioprocess and Biosystems Engineering"
DOI: 10.1007/s00449-017-1736-8
Abstract: In a recent study, Satar et al. (Bioprocess Biosyst Eng 39:807–814, 2016) have reported “the synthesis and characterization of graphene for the immobilization of β-galactosidase for improved galacto-oligosaccharide (GOS) production”. There are several issues in… read more here.
Keywords: production; comment elucidating; graphene; elucidating binding ... See more keywords
In Vitro Screening of Indigenous Lactobacillus Isolates for Selecting Organisms with Better Health-Promoting Attributes
Sign Up to like & getrecommendations! Published in 2018 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-018-2709-3
Abstract: Lactobacilli have several attributes that provide health benefits to the host. The aim of this study was to screen indigenous lactobacilli from human gut and fermented foods for such attributes as production of β- and… read more here.
Keywords: cholesterol; galactosidase; lactobacillus isolates; gpi ... See more keywords
Affecting parameters on fabrication of β-D-galactosidase immobilized chitosan/poly (vinyl alcohol) electrospun nanofibers.
Sign Up to like & getrecommendations! Published in 2018 at "Carbohydrate polymers"
DOI: 10.1016/j.carbpol.2018.07.096
Abstract: β-galactosidase was successfully immobilized onto chitosan (CS)/polyvinyl alcohol (PVA) blend nanofibers through electrospinning. The effects of both polymer solution parameters and the electrospinning process conditions on the morphology of nanofibers and enzyme activity were studied.… read more here.
Keywords: galactosidase immobilized; parameters fabrication; alcohol; galactosidase ... See more keywords
Colorimetric enumeration of bacterial contamination in water based on β-galactosidase gold nanoshell activity.
Sign Up to like & getrecommendations! Published in 2017 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2017.01.006
Abstract: In this study we report a method for the rapid and sensitive estimation of bacterial cell concentration in solution based on a colorimetric enzyme/gold nanoshells conjugate system. The CTAB capped gold nanoshells are electrostatically attracted… read more here.
Keywords: colorimetric enumeration; bacterial cell; galactosidase; gold nanoshells ... See more keywords
Production of galacto-oligosaccharides from whey permeate using β-galactosidase immobilized on functionalized glass beads.
Sign Up to like & getrecommendations! Published in 2018 at "Food chemistry"
DOI: 10.1016/j.foodchem.2018.01.068
Abstract: The conversion of whey permeates to galacto-oligosaccharides (GOS) was studied by the enzymatic action of β-galactosidase from Aspergillus oryzae in a continuous flow packed-bed reactor. A novel method of enzyme immobilization involving covalent immobilization of… read more here.
Keywords: glass beads; glass; enzyme; galactosidase ... See more keywords
Effect of biopolymer matrices on lactose hydrolysis by enzymatically active hydrogel and aerogels loaded with β-galactosidase nanoflowers
Sign Up to like & getrecommendations! Published in 2021 at "Food Hydrocolloids"
DOI: 10.1016/j.foodhyd.2020.106220
Abstract: Abstract In this work, enzymatically active polysaccharide-based hydrogels and aerogels have been developed. To this end, a thermostable β-galactosidase (TmLac) enzyme from Thermotoga maritima embedded in nanoflowers’ format was used to evaluate the capacity of… read more here.
Keywords: enzymatically active; hydrogel; agar; hydrogel capsules ... See more keywords
Characterization of a high performance α-galactosidase from Irpex lacteus and its usage in removal of raffinose family oligosaccharides from soymilk.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.04.060
Abstract: Raffinose family oligosaccharides (RFOs) negatively affect nutritional value of legume-derived food and feed. It has been challenging to develop a high performance α-galactosidase excelled on catalytic efficiency, thermostability, pH stability and protease-resistance that could efficiently… read more here.
Keywords: family oligosaccharides; raffinose family; family; galactosidase ... See more keywords