Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0868-5_11
Abstract: Budding yeast Saccharomyces cerevisiae has become a model eukaryotic microorganism for targeted genomic manipulation due to its efficient homologous recombination. A few genomic loci, including rDNA, Delta, and Ty1, can be utilized to introduce variable… read more here.
Keywords: copy; gate assembly; genetic elements; complex genetic ... See more keywords
Golden Gate assembly with a bi-directional promoter (GBid): A simple, scalable method for phage display Fab library creation
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DOI: 10.1038/s41598-020-59745-2
Abstract: Fabs offer an attractive platform for monoclonal antibody discovery/engineering, but library construction can be cumbersome. We report a simple method – Golden Gate assembly with a bi-directional promoter (GBid) – for constructing phage display Fab… read more here.
Keywords: promoter; phage display; gbid; directional promoter ... See more keywords
Golden Gate Assembly of Aerobic and Anaerobic Microbial Bioreporters.
Sign Up to like & getrecommendations! Published in 2021 at "Applied and environmental microbiology"
DOI: 10.1128/aem.01485-21
Abstract: Microbial bioreporters provide direct insight into cellular processes by producing a quantifiable signal dictated by reporter gene expression. The core of a bioreporter is a genetic circuit in which a reporter gene (or operon) is… read more here.
Keywords: mercury arsenic; biology; microbial bioreporters; gate assembly ... See more keywords
Golden Gate assembly of BioBrick-compliant parts using Type II restriction endonucleases.
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DOI: 10.2144/btn-2021-0083
Abstract: Aims: New methods of DNA recombination that capture the principal advantages of the BioBrick standard (ease of design) and Golden Gate assembly (decreased labor) are demonstrated here. Methods & materials: Both methods employ DNA methyltransferase… read more here.
Keywords: golden gate; restriction endonucleases; gate assembly; assembly ... See more keywords
Easy method for six-fragment Golden Gate Assembly of modular vectors.
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DOI: 10.2144/btn-2022-0041
Abstract: Efficient cloning techniques are a requirement for synthetic biology. This study provides a simplified cloning method based on Golden Gate Assembly that can be used for rapid vector construction. The building of multiple expression vectors… read more here.
Keywords: easy method; golden gate; gate assembly; method six ... See more keywords