A platform method for plasmid isoforms analysis by capillary gel electrophoresis
Sign Up to like & getrecommendations! Published in 2022 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202100343
Abstract: In the production of novel biological products, plasmids are often engineered into delivery vectors for target genes, which can be used directly as vaccines or as intermediate products for gene/cell therapy. Plasmid DNA exists in… read more here.
Keywords: gel electrophoresis; plasmid; platform method; method ... See more keywords
Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of separation science"
DOI: 10.1002/jssc.201900495
Abstract: Shotgun proteomics based on peptide fractionation via liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation via electrophoresis was the main tool. Nonetheless, during… read more here.
Keywords: electrophoresis; sodium dodecyl; dodecyl sulfate; gel electrophoresis ... See more keywords
Gel electrophoresis/electroelution sorting fractionator combined with filter aided sample preparation for deep proteomic analysis.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of separation science"
DOI: 10.1002/jssc.202100992
Abstract: Sample preparation and protein fractionation are important issues for proteomic studies. Protein extraction procedures strongly affect the performance of fractionation methods by provoking protein dispersion in several fractions. The most notable exception is the gel-based… read more here.
Keywords: sample preparation; fractionator; preparation; analysis ... See more keywords
Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0876-0_11
Abstract: The oxidation activity of the mammalian ten-eleven translocation dioxygenase (TET) on 5-methylcytosine (5mC) of DNA is usually monitored by analytical methods such as dot blotting and liquid chromatography-mass spectrometry (LC-MS). Herein, we describe a high… read more here.
Keywords: gel electrophoresis; detection tet; methylcytosine; capillary gel ... See more keywords
The Single-Cell Gel Electrophoresis Genotoxin Sensitivity Assay.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1091-6_1
Abstract: The single-cell gel electrophoresis-based genotoxin sensitivity assay (GSA) is an ex vivo approach which enables to study the impact of a variety of dietary factors, occupational exposures, and diseases on the sensitivity of humans towards… read more here.
Keywords: single cell; gel electrophoresis; sensitivity assay; cell gel ... See more keywords
Karyotyping of Fusarium oxysporum by Pulsed-Field Gel Electrophoresis and the Germ Tube Burst Method.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1795-3_3
Abstract: In fungi, karyotyping is fundamental to understanding their genome organization. It is also essential to study various genome- or chromosome-related topics such as karyotype polymorphisms and supernumerary or pathogenicity chromosomes. Here, we describe the protocols… read more here.
Keywords: gel electrophoresis; germ tube; pulsed field; tube burst ... See more keywords
Analyzing Copy Number Variation Using Pulsed-Field Gel Electrophoresis: Providing a Genetic Diagnosis for FSHD1.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6442-0_7
Abstract: The myopathy facioscapulohumeral muscular dystrophy type 1 (FSHD1) is caused by copy number variation of the D4Z4 macrosatellite repeat on chromosome 4. In unaffected individuals the number of 3.3 kb D4Z4 units varies between 8… read more here.
Keywords: copy number; number variation; gel electrophoresis; pulsed field ... See more keywords
Two-Dimensional Difference Gel Electrophoresis.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8793-1_20
Abstract: Two-dimensional difference gel electrophoresis (2D DIGE) is a modified form of 2D electrophoresis (2D E) that allows one to compare two or three protein samples simultaneously on the same gel. The proteins in each sample… read more here.
Keywords: protein; dimensional difference; gel electrophoresis; two dimensional ... See more keywords
A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8793-1_36
Abstract: We developed a multichannel gel electrophoresis system that continuously collects fractions as protein bands migrate to the bottom of gel columns. The device uses several short linear gel columns, each of a different percent acrylamide,… read more here.
Keywords: protein; gel electrophoresis; throughput protein; fraction collection ... See more keywords
Two-Dimensional Gel Electrophoresis: A Reference Protocol.
Sign Up to like & getrecommendations! Published in 2017 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-319-52479-5_13
Abstract: Two-dimensional gel electrophoresis (2DE) has been a mainstay of proteomic techniques for more than four decades. It was even in use for several years before the term proteomics was actually coined in the early 1990s.… read more here.
Keywords: dimensional gel; reference protocol; gel electrophoresis; electrophoresis reference ... See more keywords
Denaturing gradient gel electrophoresis profiles of bacteria from the saliva of twenty four different individuals form clusters that showed no relationship to the yeasts present.
Sign Up to like & getrecommendations! Published in 2017 at "Archives of oral biology"
DOI: 10.1016/j.archoralbio.2017.05.014
Abstract: OBJECTIVES The aim was to investigate the relationship between groups of bacteria identified by cluster analysis of the DGGE fingerprints and the amounts and diversity of yeast present. METHODS Bacterial and yeast populations in saliva… read more here.
Keywords: denaturing gradient; gel electrophoresis; dgge; gradient gel ... See more keywords