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   Articles with "gel electrophoresis" as a keyword



Methods comparison of two‐dimensional gel electrophoresis for host cell protein characterization

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recommendations! Published in 2024 at "Biotechnology Progress"

DOI: 10.1002/btpr.3452

Abstract: Two‐dimensional electrophoresis (2DE) is a gel‐based protein separation method based on size and charge which is commonly used for the characterization of host cell proteins (HCPs) during drug development in biotech and pharmaceutical companies. HCPs… read more here.

Keywords: method; gel; gel electrophoresis; two dimensional ... See more keywords

A platform method for plasmid isoforms analysis by capillary gel electrophoresis

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recommendations! Published in 2022 at "ELECTROPHORESIS"

DOI: 10.1002/elps.202100343

Abstract: In the production of novel biological products, plasmids are often engineered into delivery vectors for target genes, which can be used directly as vaccines or as intermediate products for gene/cell therapy. Plasmid DNA exists in… read more here.

Keywords: gel electrophoresis; plasmid; platform method; method ... See more keywords

Proper application of DNA dyes in agarose gel electrophoresis

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recommendations! Published in 2024 at "ELECTROPHORESIS"

DOI: 10.1002/elps.202400082

Abstract: Various dyes are used to visualize DNA bands in agarose gel electrophoresis (AGE) by the methods of pre‐ or post‐staining. The DNA dye user's guides generally state that the binding of the dye to DNA… read more here.

Keywords: age; dna dyes; agarose gel; gel electrophoresis ... See more keywords

Sialylation Impacts Separation of a Biotherapeutic by Capillary Gel Electrophoresis

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recommendations! Published in 2025 at "ELECTROPHORESIS"

DOI: 10.1002/elps.70006

Abstract: Monitoring of critical quality attributes (CQAs) is essential for the development of biotherapeutics. One example of a CQA is molecular fragmentation, which is often analyzed by capillary gel electrophoresis with sodium dodecyl sulfate (SDS). Sialylation… read more here.

Keywords: sds; gel electrophoresis; electrophoresis; sialylation ... See more keywords

Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation.

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recommendations! Published in 2019 at "Journal of separation science"

DOI: 10.1002/jssc.201900495

Abstract: Shotgun proteomics based on peptide fractionation via liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation via electrophoresis was the main tool. Nonetheless, during… read more here.

Keywords: electrophoresis; sodium dodecyl; dodecyl sulfate; gel electrophoresis ... See more keywords

Gel electrophoresis/electroelution sorting fractionator combined with filter aided sample preparation for deep proteomic analysis.

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recommendations! Published in 2022 at "Journal of separation science"

DOI: 10.1002/jssc.202100992

Abstract: Sample preparation and protein fractionation are important issues for proteomic studies. Protein extraction procedures strongly affect the performance of fractionation methods by provoking protein dispersion in several fractions. The most notable exception is the gel-based… read more here.

Keywords: sample preparation; fractionator; preparation; analysis ... See more keywords
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Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis.

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recommendations! Published in 2021 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-0876-0_11

Abstract: The oxidation activity of the mammalian ten-eleven translocation dioxygenase (TET) on 5-methylcytosine (5mC) of DNA is usually monitored by analytical methods such as dot blotting and liquid chromatography-mass spectrometry (LC-MS). Herein, we describe a high… read more here.

Keywords: gel electrophoresis; detection tet; methylcytosine; capillary gel ... See more keywords

The Single-Cell Gel Electrophoresis Genotoxin Sensitivity Assay.

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recommendations! Published in 2021 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-1091-6_1

Abstract: The single-cell gel electrophoresis-based genotoxin sensitivity assay (GSA) is an ex vivo approach which enables to study the impact of a variety of dietary factors, occupational exposures, and diseases on the sensitivity of humans towards… read more here.

Keywords: single cell; gel electrophoresis; sensitivity assay; cell gel ... See more keywords
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Karyotyping of Fusarium oxysporum by Pulsed-Field Gel Electrophoresis and the Germ Tube Burst Method.

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recommendations! Published in 2022 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-1795-3_3

Abstract: In fungi, karyotyping is fundamental to understanding their genome organization. It is also essential to study various genome- or chromosome-related topics such as karyotype polymorphisms and supernumerary or pathogenicity chromosomes. Here, we describe the protocols… read more here.

Keywords: gel electrophoresis; germ tube; pulsed field; tube burst ... See more keywords

Analyzing Copy Number Variation Using Pulsed-Field Gel Electrophoresis: Providing a Genetic Diagnosis for FSHD1.

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recommendations! Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-6442-0_7

Abstract: The myopathy facioscapulohumeral muscular dystrophy type 1 (FSHD1) is caused by copy number variation of the D4Z4 macrosatellite repeat on chromosome 4. In unaffected individuals the number of 3.3 kb D4Z4 units varies between 8… read more here.

Keywords: copy number; number variation; gel electrophoresis; pulsed field ... See more keywords

Two-Dimensional Difference Gel Electrophoresis.

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recommendations! Published in 2019 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-8793-1_20

Abstract: Two-dimensional difference gel electrophoresis (2D DIGE) is a modified form of 2D electrophoresis (2D E) that allows one to compare two or three protein samples simultaneously on the same gel. The proteins in each sample… read more here.

Keywords: protein; dimensional difference; gel electrophoresis; two dimensional ... See more keywords