Lighting Up Gene Activation in Living Drosophila Embryos.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9674-2_5
Abstract: With its rapid development, ease of collection, and the presence of a unique layer of nuclei located close to the surface, the Drosophila syncytial embryo is ideally suited to study the establishment of gene expression… read more here.
Keywords: drosophila embryos; living drosophila; lighting gene; gene ... See more keywords
One-two punch mechanism of gene repression: a fresh perspective on gene regulation
Sign Up to like & getrecommendations! Published in 2017 at "Current Genetics"
DOI: 10.1007/s00294-017-0793-5
Abstract: Cellular differentiation depends on temporally controlled waves of gene activation and inactivation that ultimately transform one cell type into another. It is well established that transcription factor cascades coordinate the timely activation of gene expression… read more here.
Keywords: gene repression; mechanism; repression; gene ... See more keywords
In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation
Sign Up to like & getrecommendations! Published in 2017 at "Cell"
DOI: 10.1016/j.cell.2017.10.025
Abstract: Current genome-editing systems generally rely on inducing DNA double-strand breaks (DSBs). This may limit their utility in clinical therapies, as unwanted mutations caused by DSBs can have deleterious effects. CRISPR/Cas9 system has recently been repurposed… read more here.
Keywords: crispr cas9; target; activation; gene activation ... See more keywords
Robust, Durable Gene Activation In Vivo via mRNA-Encoded Activators.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.1c10631
Abstract: Programmable control of gene expression via nuclease-null Cas9 fusion proteins has enabled the engineering of cellular behaviors. Here, both transcriptional and epigenetic gene activation via synthetic mRNA and lipid nanoparticle delivery was demonstrated in vivo.… read more here.
Keywords: durable gene; gene activation; activation vivo; gene ... See more keywords
A nuclear redox sensor modulates gene activation and var switching in Plasmodium falciparum
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2201247119
Abstract: Significance The molecular mechanisms that regulate the “choice” for singular gene activation among multicopy gene families remains an unsolved mystery in eukaryotic gene expression. Immune evasion of Plasmodium falciparum, the parasite responsible for the deadliest… read more here.
Keywords: gene activation; redox sensor; plasmodium falciparum; gene ... See more keywords
Ep300 sequestration to functionally distinct glucocorticoid receptor binding loci underlie rapid gene activation and repression
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac488
Abstract: Abstract The rapid transcriptional response to the transcription factor, glucocorticoid receptor (GR), including gene activation or repression, is mediated by the spatial association of genes with multiple GR binding sites (GBSs) over large genomic distances.… read more here.
Keywords: gene activation; activation repression; gene; glucocorticoid receptor ... See more keywords
Using average transcription level to understand the regulation of stochastic gene activation
Sign Up to like & getrecommendations! Published in 2022 at "Royal Society Open Science"
DOI: 10.1098/rsos.211757
Abstract: Gene activation is a random process, modelled as a framework of multiple rate-limiting steps listed sequentially, in parallel or in combination. Together with suitably assumed processes of gene inactivation, transcript birth and death, the step… read more here.
Keywords: gene activation; using average; transcription; level ... See more keywords
The pioneer factor hypothesis is not necessary to explain ectopic liver gene activation.
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.73358
Abstract: The Pioneer Factor Hypothesis (PFH) states that pioneer factors (PFs) are a subclass of transcription factors (TFs) that bind to and open inaccessible sites and then recruit non-pioneer factors (nonPFs) that activate batteries of silent… read more here.
Keywords: gene activation; factor hypothesis; pioneer factor; pioneer ... See more keywords