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   Articles with "gene assembly" as a keyword



RoboMoClo: A Robotics-Assisted Modular Cloning Framework for Multiple Gene Assembly in Biofoundry.

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recommendations! Published in 2022 at "ACS synthetic biology"

DOI: 10.1021/acssynbio.1c00628

Abstract: Efficient and versatile DNA assembly frameworks have had an impact on promoting synthetic biology to build complex biological systems. To accelerate system development, laboratory automation (or biofoundry) provides an opportunity to construct organisms and DNA… read more here.

Keywords: robotics; multiple gene; robomoclo; gene ... See more keywords

Simple and Rapid Non-ribosomal Peptide Synthetase Gene Assembly Using the SEAM-OGAB Method.

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recommendations! Published in 2022 at "ACS synthetic biology"

DOI: 10.1021/acssynbio.2c00565

Abstract: Recombination of biosynthetic gene clusters including those of non-ribosomal peptide synthetases (NRPSs) is essential for understanding the mechanisms of biosynthesis. Due to relatively huge gene cluster sizes ranging from 10 to 150 kb, the prevalence… read more here.

Keywords: gene assembly; seam ogab; non ribosomal; ribosomal peptide ... See more keywords

Automated Construction of a Yeast-Based Multigene Library via Homologous Recombination in a Biofoundry Workflow.

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recommendations! Published in 2025 at "ACS synthetic biology"

DOI: 10.1021/acssynbio.4c00812

Abstract: Efficiently building metabolic pathways via multigene assembly has long been constrained by the limitations of traditional cloning techniques, necessitating a breakthrough in gene assembly methods. Notably, various in vitro gene assembly methods have been developed… read more here.

Keywords: gene assembly; multigene library; biology; construction ... See more keywords

A depth-first search algorithm for oligonucleotide design in gene assembly

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recommendations! Published in 2022 at "Frontiers in Genetics"

DOI: 10.3389/fgene.2022.1023092

Abstract: When synthesizing a gene with a long DNA sequence, it is usually necessary to divide it into several fragments. Based on these fragments, a set of oligonucleotides for gene assembly is produced. Each oligonucleotide is… read more here.

Keywords: gene assembly; search algorithm; first search; gene ... See more keywords