Synthetic gene circuits as tools for drug discovery.
Sign Up to like & getrecommendations! Published in 2021 at "Trends in biotechnology"
DOI: 10.1016/j.tibtech.2021.06.007
Abstract: Within mammalian systems, there exists enormous opportunity to use synthetic gene circuits to enhance phenotype-based drug discovery, to map the molecular origins of disease, and to validate therapeutics in complex cellular systems. While drug discovery… read more here.
Keywords: drug discovery; drug; synthetic gene; gene circuits ... See more keywords
Two-Layered Microfluidic Devices for High-Throughput Dynamic Analysis of Synthetic Gene Circuits in E. coli.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00307
Abstract: Escherichia coli is a common chassis for synthetic gene circuit studies. In addition to the dose-response of synthetic gene circuits, the analysis of dynamic responses is also an important part of the future design of… read more here.
Keywords: synthetic gene; two layered; layered microfluidic; analysis ... See more keywords
A framework for the modular and combinatorial assembly of synthetic gene circuits.
Sign Up to like & getrecommendations! Published in 2019 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.9b00174
Abstract: Synthetic gene circuits emerge from iterative design-build-test cycles. Most commonly, the time-limiting step is the circuit construction process. Here, we present a hierarchical cloning scheme based on the widespread Gibson assembly method and make the… read more here.
Keywords: circuit; modular combinatorial; gene circuits; combinatorial assembly ... See more keywords
Designing cell function: assembly of synthetic gene circuits for cell biology applications
Sign Up to like & getrecommendations! Published in 2018 at "Nature Reviews Molecular Cell Biology"
DOI: 10.1038/s41580-018-0024-z
Abstract: Synthetic biology is the discipline of engineering application-driven biological functionalities that were not evolved by nature. Early breakthroughs of cell engineering, which were based on ectopic (over)expression of single sets of transgenes, have already had… read more here.
Keywords: medicine; cell biology; biology; gene circuits ... See more keywords
Cooperating on synthetic gene circuits
Sign Up to like & getrecommendations! Published in 2019 at "Nature Biotechnology"
DOI: 10.1038/s41587-019-0182-3
Abstract: Biological gene networks coordinate complex behaviors through the interactions of activators and repressors with DNA. In eukaryotic gene networks, cooperative interactions between transcription factor and cofactors affect promoter binding and convert analog inputs into digital… read more here.
Keywords: engineering; transcription; circuit; gene circuits ... See more keywords
CRISPR-associated type V proteins as a tool for controlling mRNA stability in S. cerevisiae synthetic gene circuits
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac1270
Abstract: Abstract Type V-A CRISPR-(d)Cas system has been used in multiplex genome editing and transcription regulation in both eukaryotes and prokaryotes. However, mRNA degradation through the endonuclease activity of Cas12a has never been studied. In this… read more here.
Keywords: mrna stability; mrna degradation; gene circuits; synthetic gene ... See more keywords
Synthetic Gene Circuits: Design, Implement, and Apply
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the IEEE"
DOI: 10.1109/jproc.2021.3134169
Abstract: Synthetic biologists engineered the first genetic toggle switch and clock at the turn of the 21st century. Since the development of these first gene circuits, methods for circuit design and construction have improved dramatically, narrowing… read more here.
Keywords: implement apply; circuits design; synthetic gene; design implement ... See more keywords
Synthetic gene circuits for preventing disruption of the circadian clock due to interleukin-1–induced inflammation
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abj8892
Abstract: The circadian clock regulates tissue homeostasis through temporal control of tissue-specific clock-controlled genes. In articular cartilage, disruptions in the circadian clock are linked to a procatabolic state. In the presence of inflammation, the cartilage circadian… read more here.
Keywords: inflammation; circadian clock; synthetic gene; cartilage ... See more keywords
Synthetic gene circuits take root
Sign Up to like & getrecommendations! Published in 2022 at "Science"
DOI: 10.1126/science.add6805
Abstract: Description Complex spatial patterns of gene expression are engineered in plants to modulate root morphology A long-standing aim of synthetic biology has been to engineer genetic circuits that are able to confer prescribed spatiotemporal patterns… read more here.
Keywords: synthetic gene; circuits take; take root; gene ... See more keywords
Exploiting Information and Control Theory for Directing Gene Expression in Cell Populations
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.869509
Abstract: Microbial populations can adapt to adverse environmental conditions either by appropriately sensing and responding to the changes in their surroundings or by stochastically switching to an alternative phenotypic state. Recent data point out that these… read more here.
Keywords: information; control; control theory; gene ... See more keywords