Super‐killer CTLs are generated by single gene deletion of Bach2
Sign Up to like & getrecommendations! Published in 2022 at "European Journal of Immunology"
DOI: 10.1002/eji.202249797
Abstract: Bach2 codes for a transcriptional regulator exerting major influences on T cell‐mediated immune regulation. Effector CTLs derived from in vitro activation of murine CD8+ T cells showed increased proliferative and cytolytic capacity in the absence… read more here.
Keywords: ctls; bach2; super killer; gene deletion ... See more keywords
Golden Gate vectors for efficient gene fusion and gene deletion in diverse filamentous fungi
Sign Up to like & getrecommendations! Published in 2020 at "Current Genetics"
DOI: 10.1007/s00294-020-01143-2
Abstract: The cloning of plasmids can be time-consuming or expensive. Yet, cloning is a prerequisite for many standard experiments for the functional analysis of genes, including the generation of deletion mutants and the localization of gene… read more here.
Keywords: gate vectors; deletion; gene deletion; gene ... See more keywords
Gene deletion and constitutive expression of the pectate lyase gene 1 (MoPL1) lead to diminished virulence of Magnaporthe oryzae
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Microbiology"
DOI: 10.1007/s12275-022-1074-7
Abstract: Phytopathogenic fungi are known to secrete specific proteins which act as virulence factors and promote host colonization. Some of them are enzymes with plant cell wall degradation capability, like pectate lyases (Pls). In this work,… read more here.
Keywords: virulence; magnaporthe oryzae; gene deletion; gene ... See more keywords
Plasmodium falciparum isolate with histidine-rich protein 2 gene deletion from Nyala City, Western Sudan
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-69756-8
Abstract: In remote areas of malaria-endemic countries, rapid diagnostic tests (RDTs) have dramatically improved parasitological confirmation of suspected malaria cases, especially when skilled microscopists are not available. This study was designed to determine the frequency of… read more here.
Keywords: microscopy; gene deletion; plasmodium falciparum; gene ... See more keywords
Gene Deletion in Barley Mediated by LTR-retrotransposon BARE
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep43766
Abstract: A poly-row branched spike (prbs) barley mutant was obtained from soaking a two-rowed barley inflorescence in a solution of maize genomic DNA. Positional cloning and sequencing demonstrated that the prbs mutant resulted from a 28 kb… read more here.
Keywords: barley; gene deletion; gene; deletion barley ... See more keywords
Introduction of a Recombineering Oligonucleotide and a CRISPR-Cas9 Gene-Targeting Plasmid into Staphylococcus ureus for Generating a Gene-Deletion Strain.
Sign Up to like & getrecommendations! Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107921
Abstract: Gene deletions can be generated in Staphylococcus aureus using recombineering in combination with a CRISPR-Cas9 counterselection approach. The method involves first designing the recombineering oligonucleotides and generating the relevant plasmids, and then introducing these elements… read more here.
Keywords: crispr cas9; deletion; gene targeting; strain ... See more keywords
Construction of a Staphylococcus aureus Gene-Deletion Allelic-Exchange Plasmid by Gibson Assembly and Recovery in Escherichia coli.
Sign Up to like & getrecommendations! Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107946
Abstract: We present a protocol for the generation of a gene-deletion allelic-exchange plasmid and its recovery in Escherichia coli for the purpose of constructing an in-frame gene deletion in Staphylococcus aureus Here, we present detailed methodologies… read more here.
Keywords: plasmid; gene; gene deletion; allelic exchange ... See more keywords
A Seamless Gene Deletion Method and Its Application for Regulation of Higher Alcohols and Ester in Baijiu Saccharomyces cerevisiae
Sign Up to like & getrecommendations! Published in 2019 at "BioMed Research International"
DOI: 10.1155/2019/6723849
Abstract: The security of engineering Saccharomyces cerevisiae is becoming more focused on industrial production in consideration of the public concern regarding genetically modified organisms. In this work, a rapid and highly efficient system for seamless gene… read more here.
Keywords: saccharomyces cerevisiae; deletion method; deletion; gene deletion ... See more keywords
Systematic review of the status of pfhrp2 and pfhrp3 gene deletion, approaches and methods used for its estimation and reporting in Plasmodium falciparum populations in Africa: review of published studies 2010–2019
Sign Up to like & getrecommendations! Published in 2019 at "Malaria Journal"
DOI: 10.1186/s12936-019-2987-4
Abstract: BackgroundMalaria rapid diagnostic tests based on histidine-rich protein-2 have played a vital role in improving malaria case management and surveillance particularly in Africa, where Plasmodium falciparum is predominant. However, their usefulness has been threatened by… read more here.
Keywords: pfhrp2 pfhrp3; deletion; gene deletion; review ... See more keywords
Hypertrophic pyloric stenosis masked by kidney failure in a male infant with a contiguous gene deletion syndrome at Xp22.31 involving the steroid sulfatase gene: case report
Sign Up to like & getrecommendations! Published in 2022 at "Italian Journal of Pediatrics"
DOI: 10.1186/s13052-022-01218-5
Abstract: Background Contiguous gene deletion syndrome at Xp22.3 resulting in nullisomy in males or Turner syndrome patients typically encompasses the steroid sulfatase gene ( STS ) and contiguously located other genes expanding the phenotype. In large… read more here.
Keywords: deletion syndrome; syndrome xp22; contiguous gene; gene ... See more keywords
Ildr1 gene deletion protects against diet-induced obesity and hyperglycemia
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0270329
Abstract: Objective Immunoglobulin-like Domain-Containing Receptor 1 (ILDR1) is expressed on nutrient sensing cholecystokinin-positive enteroendocrine cells of the gastrointestinal tract and it has the unique ability to induce fat-mediated CCK secretion. However, the role of ILDR1 in… read more here.
Keywords: deletion; gene deletion; ildr1 gene; tolerance ... See more keywords