Efficient markerless gene deletions in Pseudomonas protegens Pf-5 using a upp-based counterselective system
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Letters"
DOI: 10.1007/s10529-019-02772-5
Abstract: ObjectivesDeveloping a counterselective system for efficient markerless gene deletions in biocontrol strain P. protegens Pf-5.ResultsWe successfully implemented a markerless deletion of upp in Pf-5 to obtain the 5-FU resistant strain Pf5139. With this strain, we… read more here.
Keywords: gene deletions; gene; markerless gene; counterselective system ... See more keywords
Effect of deletions in the α-globin gene on the phenotype severity of β-thalassemia
Sign Up to like & getrecommendations! Published in 2022 at "Hemoglobin"
DOI: 10.1080/03630269.2022.2088381
Abstract: Abstract Thalassemia is the most common inherited hemoglobinopathy worldwide. Variation of clinical symptoms in this hemoglobinopathy entails differences in disease-onset and transfusion requirements. The aim of this study was to investigate the role of α-globin… read more here.
Keywords: thal subjects; gene deletions; thalassemia; severity ... See more keywords
Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019
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DOI: 10.1186/s12936-020-03362-x
Abstract: Histidine-rich protein-2 (HRP2)-based rapid diagnostic tests (RDTs) are the only RDTs recommended for malaria diagnosis in Uganda. However, the emergence of Plasmodium falciparum histidine rich protein 2 and 3 (pfhrp2 and pfhrp3) gene deletions threatens… read more here.
Keywords: gene deletions; pfhrp2 pfhrp3; presence; gene ... See more keywords
Plasmodium falciparum pfhrp2 and pfhrp3 Gene Deletions and Relatedness to Other Global Isolates, Djibouti, 2019–2020
Sign Up to like & getrecommendations! Published in 2022 at "Emerging Infectious Diseases"
DOI: 10.3201/eid2810.220695
Abstract: Deletions of pfhrp2 and paralogue pfhrp3 (pfhrp2/3) genes threaten Plasmodium falciparum diagnosis by rapid diagnostic test. We examined 1,002 samples from suspected malaria patients in Djibouti City, Djibouti, to investigate pfhrp2/3 deletions. We performed assays… read more here.
Keywords: pfhrp2; plasmodium falciparum; gene deletions; pfhrp2 pfhrp3 ... See more keywords
Characterization of Sigma Factor Genes in Streptomyces lividans TK24 Using a Genomic Library-Based Approach for Multiple Gene Deletions
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2018.03033
Abstract: Alternative sigma factors control numerous aspects of bacterial life, including adaptation to physiological stresses, morphological development, persistence states and virulence. This is especially true for the physiologically complex actinobacteria. Here we report the development of… read more here.
Keywords: gene deletions; lividans tk24; streptomyces lividans; gene ... See more keywords