Production of selenomethionine labeled polyglycine hydrolases in Pichia pastoris.
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DOI: 10.1016/j.pep.2022.106076
Abstract: Producing recombinant proteins with incorporated selenomethionine (SeMet) facilitates solving X-ray crystallographic structures of novel proteins. Production of SeMet labeled proteins in the yeast Pichia pastoris (syn. Komagataella phaffii) is difficult because SeMet is mildly toxic,… read more here.
Keywords: pichia pastoris; expression; production; cmp ... See more keywords