Two steps are better than one: improving gene editing to treat cancer
Sign Up to like & getrecommendations! Published in 2021 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13342
Abstract: Gene editing enables scientists to make precise changes to the genome of an organism using the cell’s own ability to repair damaged DNA using a supplied DNA template. In recent years, gene editing has been… read more here.
Keywords: dna; cancer; two steps; steps better ... See more keywords
The Delivery Challenge of Genome Editing in Human Epithelia.
Sign Up to like & getrecommendations! Published in 2021 at "Advanced healthcare materials"
DOI: 10.1002/adhm.202100847
Abstract: Despite exciting advances in gene editing, their clinical translation is still hampered by the lack of delivery systems that can encapsulate and deliver gene editing tools like CRISPR-Cas9 or prime editors to the target side.… read more here.
Keywords: genome editing; gene editing; human epithelia; delivery ... See more keywords
A Versatile Nonviral Delivery System for Multiplex Gene-Editing in the Liver.
Sign Up to like & getrecommendations! Published in 2020 at "Advanced materials"
DOI: 10.1002/adma.202003537
Abstract: Recent advances in CRISPR present attractive genome-editing toolsets for therapeutic strategies at the genetic level. Here, a liposome-coated mesoporous silica nanoparticle (lipoMSN) is reported as an effective CRISPR delivery system for multiplex gene-editing in the… read more here.
Keywords: gene editing; multiplex gene; delivery system; editing liver ... See more keywords
Chemical Control of CRISPR Gene Editing via Conditional Diacylation Crosslinking of Guide RNAs
Sign Up to like & getrecommendations! Published in 2023 at "Advanced Science"
DOI: 10.1002/advs.202206433
Abstract: Conditional control of RNA structure and function has emerged as an effective toolkit. Here, a strategy based on a one‐step introduction of diacylation linkers and azide groups on the 2′‐OH of RNA is advance. Selected… read more here.
Keywords: gene editing; chemical control; guide rnas; diacylation ... See more keywords
Gulo gene locus, a new gene editing locus for mammalian cells
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202100493
Abstract: Transgene technology is valuable and helpful in many fields, including basic medical and biological research, biotechnology, and therapy. Recent advances in targeting technology accelerate the production of transgenic plants and animals and the application for… read more here.
Keywords: locus new; gene editing; gulo gene; gene locus ... See more keywords
Chemical Modification and Transformation Strategies of Guide RNAs in CRISPR-Cas9 Gene Editing Systems.
Sign Up to like & getrecommendations! Published in 2021 at "ChemPlusChem"
DOI: 10.1002/cplu.202000785
Abstract: The CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR-associated protein 9) is a most powerful tool and has been widely used in gene editing and gene regulation since its discovery. However, wild-type CRISPR-Cas9 suffers from… read more here.
Keywords: guide rnas; gene editing; crispr cas9; gene ... See more keywords
Use of mitochondrial sequencing to detect gene doping in horses via gene editing and somatic cell nuclear transfer.
Sign Up to like & getrecommendations! Published in 2022 at "Drug testing and analysis"
DOI: 10.1002/dta.3267
Abstract: Gene editing and subsequent cloning techniques offer great potential not only in genetic disease correction in domestic animals, but also in livestock production by enhancement of desirable traits. The existence of the technology, however, leaves… read more here.
Keywords: mitochondrial sequencing; nuclear transfer; cell nuclear; somatic cell ... See more keywords
Gene editing provides a tool to investigate genes involved in reproduction of pigs.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular reproduction and development"
DOI: 10.1002/mrd.23620
Abstract: CRISPR-Cas9 gene editing technology provides a method to generate loss-of-function studies to investigate, in vivo, the specific role of specific genes in regulation of reproduction. With proper design and selection of guide RNAs (gRNA) designed… read more here.
Keywords: crispr cas9; gene editing; cas9 gene; editing technology ... See more keywords
Developments in Hematopoietic Stem Cell Expansion and Gene Editing Technologies.
Sign Up to like & getrecommendations! Published in 2018 at "Advances in experimental medicine and biology"
DOI: 10.1007/5584_2017_114
Abstract: Hematopoietic stem cells (HSCs) are rare cells, which housed in the adult bone marrow. They maintain all types of differentiated blood cells throughout life. Due to limited availability of HSCs for transplantation, treatment of various… read more here.
Keywords: gene editing; expansion; expansion gene; editing technologies ... See more keywords
Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/7651_2018_115
Abstract: The clustered regularly interspaced, short palindromic repeats (CRISPR)-Cas9 system functions like an adaptive immune system in a variety of microbes and has recently been engineered as a powerful tool for manipulating genomic sequences in a… read more here.
Keywords: skin stem; stem cells; vivo gene; gene editing ... See more keywords
Gene Editing in Human Induced Pluripotent Stem Cells Using Doxycycline-Inducible CRISPR-Cas9 System.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/7651_2021_348
Abstract: Induced pluripotent stem cells (iPSCs) generated from patients are a valuable tool for disease modelling, drug screening, and studying the functions of cell/tissue-specific genes. However, for this research, isogenic iPSC lines are important for comparison… read more here.
Keywords: gene editing; pluripotent stem; induced pluripotent; gene ... See more keywords