Electroporation-Induced Stress Response and Its Effect on Gene Electrotransfer Efficacy: In Vivo Imaging and Numerical Modeling
Sign Up to like & getrecommendations! Published in 2019 at "IEEE Transactions on Biomedical Engineering"
DOI: 10.1109/tbme.2019.2894659
Abstract: Objective: Skin is an attractive target tissue for gene transfer due to its size, accessibility, and its immune competence. One of the promising delivery methods is gene delivery by means of electroporation (EP), i.e., gene… read more here.
Keywords: gene electrotransfer; gene; induced stress; stress response ... See more keywords
Abstract 5122: Electrochemotherapy and IL-12 gene electrotransfer in treatment of mast cell tumors in companion dogs
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-5122
Abstract: When cells or tissues are exposed to an electric field, structural changes can be induced in the cell membrane, allowing molecules to flow into the cells. This condition is useful for delivering cytotoxic drugs whose… read more here.
Keywords: cell; companion dogs; ect get; gene electrotransfer ... See more keywords
Treatment of Skin Tumors with Intratumoral Interleukin 12 Gene Electrotransfer in the Head and Neck Region: A First-in-human Clinical Trial Protocol
Sign Up to like & getrecommendations! Published in 2022 at "Radiology and Oncology"
DOI: 10.2478/raon-2022-0021
Abstract: Abstract Background Immune therapies are currently under intensive investigation providing in many cases excellent responses in different tumors. Other possible approach for immunotherapy is a targeted intratumoral delivery of interleukin 12 (IL-12), a cytokine with… read more here.
Keywords: gene electrotransfer; human clinical; clinical trial; treatment ... See more keywords
In Vivo and Ex Vivo Gene Electrotransfer in Ophthalmological Disorders
Sign Up to like & getrecommendations! Published in 2022 at "Biomedicines"
DOI: 10.3390/biomedicines10081889
Abstract: The aim of this document is to present an overview of gene electrotransfer in ophthalmological disorders. In order to ensure an adequate variety of the assessed studies, several electronic databases were considered and studies published… read more here.
Keywords: ophthalmological disorders; gene electrotransfer; vivo gene; vivo vivo ... See more keywords
Systemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model
Sign Up to like & getrecommendations! Published in 2022 at "Biomedicines"
DOI: 10.3390/biomedicines10112687
Abstract: Non-adherent cells are difficult to transfect with chemical-mediated delivery methods. Electroporation is an attractive strategy to transfer the molecules of interest into suspension cells. Care must be taken with the viability of the transfected cells… read more here.
Keywords: systemic optimization; cell; hard transfect; gene electrotransfer ... See more keywords
Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate
Sign Up to like & getrecommendations! Published in 2022 at "Pharmaceutics"
DOI: 10.3390/pharmaceutics14091959
Abstract: Gene electrotransfer is one of the main non-viral methods for intracellular delivery of plasmid DNA, wherein pulsed electric fields are used to transiently permeabilize the cell membrane, allowing enhanced transmembrane transport. By localizing the electric… read more here.
Keywords: cell culture; cell; culture inserts; gene electrotransfer ... See more keywords
Gene Electrotransfer Efficiency in 2D and 3D Cancer Cell Models Using Different Electroporation Protocols: A Comparative Study
Sign Up to like & getrecommendations! Published in 2023 at "Pharmaceutics"
DOI: 10.3390/pharmaceutics15031004
Abstract: Electroporation, a method relying on a pulsed electric field to induce transient cell membrane permeabilization, can be used as a non-viral method to transfer genes in vitro and in vivo. Such transfer holds great promise… read more here.
Keywords: cell; electroporation; gene electrotransfer; permeabilization ... See more keywords
Electrochemotherapy Plus IL-2+IL-12 Gene Electrotransfer in Spontaneous Inoperable Stage III–IV Canine Oral Malignant Melanoma
Sign Up to like & getrecommendations! Published in 2023 at "Vaccines"
DOI: 10.3390/vaccines11061033
Abstract: Electrochemotherapy (ECT) is a standard of care in veterinary and human oncology. The treatment induces a well-characterized local immune response which is not able to induce a systemic response. In this retrospective cohort study, we… read more here.
Keywords: gene electrotransfer; oral malignant; stage iii; response ... See more keywords