Assessing gene function in human B cells: CRISPR/Cas9‐based gene editing and mRNA‐based gene expression in healthy and tumor cells
Sign Up to like & getrecommendations! Published in 2022 at "European Journal of Immunology"
DOI: 10.1002/eji.202149784
Abstract: Robust protocols for genetic manipulation are highly needed to facilitate study of gene function in human B cells, both in settings of health and disease. B cells are notoriously hard to manipulate, being especially fragile… read more here.
Keywords: based gene; function human; human cells; gene function ... See more keywords
Host-Induced Gene Silencing (HIGS) for Elucidating Puccinia Gene Function in Wheat.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8724-5_12
Abstract: Biotrophic fungi (Puccinia spp.) cause devastating diseases of wheat and other cereal species globally. The function of large repertories of genes from Puccinia spp. still needs to be discovered to understand the infection process of… read more here.
Keywords: induced gene; silencing higs; host induced; gene function ... See more keywords
Mutational analysis of gene function in the Anaplasmataceae: Challenges and perspectives.
Sign Up to like & getrecommendations! Published in 2019 at "Ticks and tick-borne diseases"
DOI: 10.1016/j.ttbdis.2018.11.006
Abstract: Mutational analysis is an efficient approach to identifying microbial gene function. Until recently, lack of an effective tool for Anaplasmataceae yielding reproducible results has created an obstacle to functional genomics, because surrogate systems, e.g., ectopic… read more here.
Keywords: analysis; gene function; mutational analysis; analysis gene ... See more keywords
Gene function prediction based on Gene Ontology Hierarchy Preserving Hashing.
Sign Up to like & getrecommendations! Published in 2019 at "Genomics"
DOI: 10.1016/j.ygeno.2018.02.008
Abstract: Gene Ontology (GO) uses structured vocabularies (or terms) to describe the molecular functions, biological roles, and cellular locations of gene products in a hierarchical ontology. GO annotations associate genes with GO terms and indicate the… read more here.
Keywords: gene; gene function; ontology; function prediction ... See more keywords
Neurodegenerative gene's function is not all about those bases.
Sign Up to like & getrecommendations! Published in 2020 at "Nature"
DOI: 10.1038/d41586-020-02382-6
Abstract: A mutation in the C9orf72 gene is the most common genetic cause of two neurodegenerative diseases. A newly identified immunological function for the C9orf72 protein points to a potential therapeutic strategy for these diseases. Loss… read more here.
Keywords: gene function; c9orf72; function; gene ... See more keywords
Translational control of gene function through optically regulated nucleic acids.
Sign Up to like & getrecommendations! Published in 2021 at "Chemical Society reviews"
DOI: 10.1039/d1cs00257k
Abstract: Translation of mRNA into protein is one of the most fundamental processes within biological systems. Gene expression is tightly regulated both in space and time, often involving complex signaling or gene regulatory networks, as most… read more here.
Keywords: nucleic acids; gene function; translational control; function ... See more keywords
Phenomics approaches to understand genetic networks and gene function in yeast
Sign Up to like & getrecommendations! Published in 2022 at "Biochemical Society Transactions"
DOI: 10.1042/bst20210285
Abstract: Over the past decade, major efforts have been made to systematically survey the characteristics or phenotypes associated with genetic variation in a variety of model systems. These so-called phenomics projects involve the measurement of ‘phenomes’,… read more here.
Keywords: genetic networks; approaches understand; phenomics approaches; gene function ... See more keywords
Network-based methods for gene function prediction.
Sign Up to like & getrecommendations! Published in 2021 at "Briefings in functional genomics"
DOI: 10.1093/bfgp/elab006
Abstract: The rapid development of high-throughput technology has generated a large number of biological networks. Network-based methods are able to provide rich information for inferring gene function. This is composed of analyzing the topological characteristics of… read more here.
Keywords: gene function; network based; network; based methods ... See more keywords
MGEGFP: a multi-view graph embedding method for gene function prediction based on adaptive estimation with GCN
Sign Up to like & getrecommendations! Published in 2022 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbac333
Abstract: In recent years, a number of computational approaches have been proposed to effectively integrate multiple heterogeneous biological networks, and have shown impressive performance for inferring gene function. However, the previous methods do not fully represent… read more here.
Keywords: function; function prediction; multi view; gene function ... See more keywords
Accurate and efficient gene function prediction using a Multi-Bacterial network.
Sign Up to like & getrecommendations! Published in 2020 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btaa885
Abstract: MOTIVATION Nearly 40% of the genes in sequenced genomes have no experimentally- or computationally-derived functional annotations. To fill this gap, we seek to develop methods for network-based gene function prediction that can integrate heterogeneous data… read more here.
Keywords: network; gene function; function prediction; prediction ... See more keywords
Virus-induced gene silencing for in planta validation of gene function in cucurbits.
Sign Up to like & getrecommendations! Published in 2022 at "Plant physiology"
DOI: 10.1093/plphys/kiac363
Abstract: Virus-induced gene silencing (VIGS) is a powerful tool for high-throughput analysis of gene function. Here, we developed the VIGS vector pCF93, from which expression of the cucumber fruit mottle mosaic virus genome is driven by… read more here.
Keywords: gene silencing; gene function; gene; virus ... See more keywords