Vacuum infiltration enhances the Agrobacterium-mediated transient transformation for gene functional analysis in persimmon (Diospyros kaki Thunb.)
Sign Up to like & getrecommendations! Published in 2019 at "Scientia Horticulturae"
DOI: 10.1016/j.scienta.2019.03.002
Abstract: Abstract An Agrobacterium-mediated transient transformation system was established for gene functional analysis in persimmon (Diospyros kaki Thunb.) utilizing sonication followed by vacuum infiltration of seedlings in vitro. The optimal vacuum conditions for agroinfiltration of persimmon… read more here.
Keywords: gene functional; vacuum infiltration; functional analysis; gene ... See more keywords
A callus transformation system for gene functional studies in soybean.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Integrative Agriculture"
DOI: 10.1016/s2095-3119(16)61621-0
Abstract: Abstract Obtaining transgenic plants is a common method for analyzing gene function. Unfortunately, stable genetic transformation is difficult to achieve, especially for plants (e.g., soybean), which are recalcitrant to genetic transformation. Transient expression systems, such… read more here.
Keywords: system; gene functional; functional studies; callus transformation ... See more keywords
Reconstruction of human protein-coding gene functional association network based on machine learning
Sign Up to like & getrecommendations! Published in 2022 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbab552
Abstract: Networks consisting of molecular interactions are intrinsically dynamical systems of an organism. These interactions curated in molecular interaction databases are still not complete and contain false positives introduced by high-throughput screening experiments. In this study,… read more here.
Keywords: network; coding gene; human protein; protein coding ... See more keywords
Highly efficient Agrobacterium rhizogenes-mediated hairy root transformation for gene functional and gene editing analysis in soybean
Sign Up to like & getrecommendations! Published in 2021 at "Plant Methods"
DOI: 10.1186/s13007-021-00778-7
Abstract: Background Agrobacterium -mediated genetic transformation is a widely used and efficient technique for gene functional research in crop breeding and plant biology. While in some plant species, including soybean, genetic transformation is still recalcitrant and… read more here.
Keywords: system; gene functional; transformation; gene ... See more keywords
Gene Functional Networks from Time Expression Profiles: A Constructive Approach Demonstrated in Chili Pepper (Capsicum annuum L.)
Sign Up to like & getrecommendations! Published in 2023 at "Plants"
DOI: 10.3390/plants12051148
Abstract: Gene co-expression networks are powerful tools to understand functional interactions between genes. However, large co-expression networks are difficult to interpret and do not guarantee that the relations found will be true for different genotypes. Statistically… read more here.
Keywords: time; time expression; expression profiles; expression ... See more keywords