BMP‐2/TGF‐β1 gene insertion into ligament‐derived stem cells sheet promotes tendon–bone healing in a mouse
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DOI: 10.1002/biot.202200470
Abstract: Bone morphogenetic protein‐2 (BMP‐2) and transforming growth factor‐β1 (TGF‐β1) reportedly induce the osteogenic and tenogenic differentiation of anterior cruciate ligament (ACL)‐derived stem cells (LDSCs), respectively. However, few studies have investigated the effect of BMP‐2/TGF‐β1 on… read more here.
Keywords: bmp tgf; derived stem; gene insertion; bone ... See more keywords
Intron-targeted gene insertion in rice using CRISPR/Cas9: A case study of the Pi-ta gene
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DOI: 10.1016/j.cj.2019.03.006
Abstract: Abstract Intron-targeted gene insertion strategy using CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated Cas9) has been shown to be a potential tool for crop genetic improvement by targeted mutagenesis or gene replacement of an elite… read more here.
Keywords: intron targeted; gene; rice; gene insertion ... See more keywords
Targeted Gene Insertion and Replacement in the Basidiomycete Ganoderma lucidum by Inactivation of Nonhomologous End Joining Using CRISPR/Cas9
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DOI: 10.1128/aem.01510-21
Abstract: Functional genomic studies in Ganoderma have been hindered by the absence of adequate genome-engineering tools. Although CRISPR/Cas9 works well for gene disruption and deletion in G. lucidum, targeted gene insertion and replacement have remained a… read more here.
Keywords: targeted gene; gene; insertion replacement; gene insertion ... See more keywords
The effects of identical nim gene-insertion sequence combinations on the expression of the nim genes and metronidazole resistance in Bacteroides fragilis strains.
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DOI: 10.2139/ssrn.4375659
Abstract: In this study we examined whether the same nim gene-insertion sequence (IS) element combinations give rise to the same expression levels as they harbor shared IS element-borne promoters. From our quantitative analysis, we found that… read more here.
Keywords: metronidazole resistance; nim gene; insertion sequence; gene insertion ... See more keywords