Evolutionarily conserved function of the even-skipped ortholog in insects revealed by gene knock-out analyses in Gryllus bimaculatus.
Sign Up to like & getrecommendations! Published in 2022 at "Developmental biology"
DOI: 10.1016/j.ydbio.2022.02.005
Abstract: Comparing the developmental mechanisms of segmentation among insects with different modes of embryogenesis provides insights on how the function of segmentation genes evolved. Functional analysis of eve by genetic mutants shows that the Drosophila pair-rule… read more here.
Keywords: insects; function; gryllus bimaculatus; gene ... See more keywords
MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-19842-2
Abstract: Gene editing nuclease represented by Cas9 efficiently generates DNA double strand breaks at the target locus, followed by repair through either the error-prone non-homologous end joining or the homology directed repair pathways. To improve Cas9’s… read more here.
Keywords: knock rates; large size; gene knock; target ... See more keywords
An efficient gene knock-in strategy using 5’-modified dsDNA donors with short homology arms
Sign Up to like & getrecommendations! Published in 2019 at "Nature chemical biology"
DOI: 10.1038/s41589-019-0432-1
Abstract: Here, we report a rapid CRISPR–Cas9-mediated gene knock-in strategy that uses Cas9 ribonucleoprotein and 5′-modified double-stranded DNA donors with 50-base-pair homology arms and achieved unprecedented 65/40% knock-in rates for 0.7/2.5 kilobase inserts, respectively, in human embryonic… read more here.
Keywords: strategy; gene knock; gene; homology arms ... See more keywords
Effects of cofD gene knock-out on the methanogenesis of Methanobrevibacter ruminantium
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DOI: 10.1186/s13568-021-01236-2
Abstract: This study aimed to investigate the effects of cofD gene knock-out on the synthesis of coenzyme F 420 and production of methane in Methanobrevibacter ruminantium ( M. ruminantium ). The experiment successfully constructed a cofD gene… read more here.
Keywords: effects cofd; gene knock; ruminantium; cofd gene ... See more keywords