CRISPR/Cas9‐mediated sheep MSTN gene knockout and promote sSMSCs differentiation
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.27474
Abstract: Myostatin (MSTN) is an important gene involved in the regulation of embryonic muscle cells and adult muscle development; it has a good application prospect in transgenic animal production by improving the yield of muscle. The… read more here.
Keywords: mstn gene; mstn; crispr cas9; gene knockout ... See more keywords
LeishGEdit: A Method for Rapid Gene Knockout and Tagging Using CRISPR-Cas9.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9210-2_9
Abstract: Postgenomic analyses of Leishmania biology benefit from rapid and precise methods for gene manipulation. Traditional methods of gene knockout or tagging by homologous recombination have limitations: they tend to be slow and require successive transfection… read more here.
Keywords: crispr cas9; knockout tagging; method; biology ... See more keywords
An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-02456-y
Abstract: Human pluripotent stem cells (hPSCs) represent a unique opportunity for understanding the molecular mechanisms underlying complex traits and diseases. CRISPR/Cas9 is a powerful tool to introduce genetic mutations into the hPSCs for loss-of-function studies. Here,… read more here.
Keywords: system; crispr cas9; gene knockout; highly efficient ... See more keywords
High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-30188-0
Abstract: Targeted mutagenesis using CRISPR/Cas9 technology has been shown to be a powerful approach to examine gene function in diverse metazoan species. One common drawback is that mixed genotypes, and thus variable phenotypes, arise in the… read more here.
Keywords: microhomology mediated; clytia; crispr cas9; gene knockout ... See more keywords
Efficient circular gene knockout system for Burkholderiales strain DSM 7029 and Mycobacterium smegmatis mc2 155.
Sign Up to like & getrecommendations! Published in 2019 at "Acta biochimica et biophysica Sinica"
DOI: 10.1093/abbs/gmz054
Abstract: Multiple gene knockouts are often employed in studies of microbial physiology and genetics. However, the selective markers that confer antibiotic resistance are generally limited, so it is necessary to remove these resistance genes before the… read more here.
Keywords: gene; knockout system; circular gene; gene knockout ... See more keywords
Gene knockout inference with variational graph autoencoder learning single-cell gene regulatory networks.
Sign Up to like & getrecommendations! Published in 2023 at "Nucleic acids research"
DOI: 10.1093/nar/gkad450
Abstract: In this paper, we introduce Gene Knockout Inference (GenKI), a virtual knockout (KO) tool for gene function prediction using single-cell RNA sequencing (scRNA-seq) data in the absence of KO samples when only wild-type (WT) samples… read more here.
Keywords: cell; variational graph; gene knockout; single cell ... See more keywords
Txnip gene knockout ameliorated HFD-induced cardiomyopathy via regulating mitochondria dynamics and fatty acid oxidation.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of cardiovascular pharmacology"
DOI: 10.1097/fjc.0000000000001414
Abstract: ABSTRACT Epidemic of obesity accelerates the increase in the number of patients with obesity cardiomyopathy. Thioredoxin interacting protein (TXNIP) has been implicated in the pathogenesis of multiple cardiovascular diseases. However, its specific role in obesity… read more here.
Keywords: txnip; hfd; gene knockout; induced cardiomyopathy ... See more keywords
Efficient Gene Knockout in Salivary Gland Epithelial Explant Cultures
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Dental Research"
DOI: 10.1177/00220345221128201
Abstract: We have developed methods to achieve efficient CRISPR-Cas9–mediated gene knockout in ex vivo mouse embryonic salivary epithelial explants. Salivary epithelial explants provide a valuable model for characterizing cell signaling, differentiation, and epithelial morphogenesis, but research… read more here.
Keywords: efficient gene; knockout salivary; salivary epithelial; gene knockout ... See more keywords
A CRISPR-del-based pipeline for complete gene knockout in human diploid cells
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cell Science"
DOI: 10.1242/jcs.260000
Abstract: The advance of CRISPR/Cas9 technology has enabled us easily to generate gene knockout cell lines by introducing insertion/deletion mutations (indels) at the target site via the error-prone non-homologous end joining repair system. Frameshift-promoting indels can… read more here.
Keywords: complete gene; gene knockout; crispr del; human diploid ... See more keywords
The gene knockout of angiotensin II type 1a receptor improves high-fat diet-induced obesity in rat via promoting adipose lipolysis
Sign Up to like & getrecommendations! Published in 2022 at "PLoS ONE"
DOI: 10.1371/journal.pone.0267331
Abstract: Aims The renin-angiotensin system (RAS) is over-activated and the serum angiotensin II (Ang II) level increased in obese patients, while their correlations were incompletely understood. This study aims to explore the role of Ang II… read more here.
Keywords: lipolysis; gene knockout; high fat; adipose lipolysis ... See more keywords
Age-dependent mechanical properties of tail tendons in wild-type and mimecan gene-knockout mice - A preliminary study.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the mechanical behavior of biomedical materials"
DOI: 10.2139/ssrn.4216645
Abstract: Mimecan, or osteoglycin, belongs to the family of small leucine-rich proteoglycans. In connective tissues mimecan is implicated in the development and maintenance of normal collagen fibrillar organization. Since collagen fibrils are responsible for tissue reinforcement,… read more here.
Keywords: gene knockout; age; mimecan gene; mechanical properties ... See more keywords