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   Articles with "gene knockout" as a keyword



CRISPR/Cas9‐mediated sheep MSTN gene knockout and promote sSMSCs differentiation

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recommendations! Published in 2018 at "Journal of Cellular Biochemistry"

DOI: 10.1002/jcb.27474

Abstract: Myostatin (MSTN) is an important gene involved in the regulation of embryonic muscle cells and adult muscle development; it has a good application prospect in transgenic animal production by improving the yield of muscle. The… read more here.

Keywords: mstn gene; mstn; crispr cas9; gene knockout ... See more keywords

LeishGEdit: A Method for Rapid Gene Knockout and Tagging Using CRISPR-Cas9.

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recommendations! Published in 2019 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-9210-2_9

Abstract: Postgenomic analyses of Leishmania biology benefit from rapid and precise methods for gene manipulation. Traditional methods of gene knockout or tagging by homologous recombination have limitations: they tend to be slow and require successive transfection… read more here.

Keywords: crispr cas9; knockout tagging; method; biology ... See more keywords

Unraveling the Molecular Landscape of SCN1A Gene Knockout in Cerebral Organoids: A Multiomics Approach Utilizing Proteomics, Lipidomics, and Transcriptomics

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recommendations! Published in 2024 at "ACS Omega"

DOI: 10.1021/acsomega.4c05039

Abstract: This study investigates the impact of sodium channel protein type 1 subunit alpha (SCN1A) gene knockout (SCN1A KO) on brain development and function using cerebral organoids coupled with a multiomics approach. From comprehensive omics analyses,… read more here.

Keywords: unraveling molecular; scn1a gene; gene knockout; multiomics approach ... See more keywords
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An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells

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recommendations! Published in 2017 at "Scientific Reports"

DOI: 10.1038/s41598-017-02456-y

Abstract: Human pluripotent stem cells (hPSCs) represent a unique opportunity for understanding the molecular mechanisms underlying complex traits and diseases. CRISPR/Cas9 is a powerful tool to introduce genetic mutations into the hPSCs for loss-of-function studies. Here,… read more here.

Keywords: system; crispr cas9; gene knockout; highly efficient ... See more keywords

High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion

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recommendations! Published in 2018 at "Scientific Reports"

DOI: 10.1038/s41598-018-30188-0

Abstract: Targeted mutagenesis using CRISPR/Cas9 technology has been shown to be a powerful approach to examine gene function in diverse metazoan species. One common drawback is that mixed genotypes, and thus variable phenotypes, arise in the… read more here.

Keywords: microhomology mediated; clytia; crispr cas9; gene knockout ... See more keywords

Optimization of gene knockout approaches and sgRNA selection in hPSCs with inducible Cas9 expression

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recommendations! Published in 2025 at "Scientific Reports"

DOI: 10.1038/s41598-025-24184-4

Abstract: CRISPR/Cas9 has been extensively used for gene knockout, enabling functional studies of genetic loss-of-function in human pluripotent stem cells (hPSCs). However, commonly used Cas9 systems usually exhibit limited and variable efficiencies, and identifying single-guide RNAs… read more here.

Keywords: sgrna selection; hpscs; cas9; gene knockout ... See more keywords

Integrating gene knockout, GC-O-MS and multivariate statistical analysis: revealing the impact of fadA and bkdB genes on the aroma characteristics of Bacillus subtilis BJ3-2 fermented soybeans

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recommendations! Published in 2025 at "RSC Advances"

DOI: 10.1039/d5ra02302e

Abstract: Fermented soybean products are an integral part of the Chinese diet, valued for their distinctive aroma. Bacillus subtilis (B. subtilis) BJ3-2 is a highly effective fermentation strain widely used in the industrial production of bacterial-type… read more here.

Keywords: bacillus subtilis; analysis; aroma; gene knockout ... See more keywords

Efficient circular gene knockout system for Burkholderiales strain DSM 7029 and Mycobacterium smegmatis mc2 155.

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recommendations! Published in 2019 at "Acta biochimica et biophysica Sinica"

DOI: 10.1093/abbs/gmz054

Abstract: Multiple gene knockouts are often employed in studies of microbial physiology and genetics. However, the selective markers that confer antibiotic resistance are generally limited, so it is necessary to remove these resistance genes before the… read more here.

Keywords: gene; knockout system; circular gene; gene knockout ... See more keywords

CRISPR-based gene knockout in the model haloarchaeon Haloferax mediterranei.

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recommendations! Published in 2025 at "FEMS microbiology letters"

DOI: 10.1093/femsle/fnaf096

Abstract: Halophilic archaea, a specialized group of extremophiles that inhabit hypersaline environments, exhibit distinctive physiological and metabolic features. Traditional genetic manipulation of these organisms, predominantly reliant on homologous recombination techniques, suffers from limitations such as complex… read more here.

Keywords: crispr based; gene knockout; crispr; gene ... See more keywords

Gene knockout inference with variational graph autoencoder learning single-cell gene regulatory networks.

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recommendations! Published in 2023 at "Nucleic acids research"

DOI: 10.1093/nar/gkad450

Abstract: In this paper, we introduce Gene Knockout Inference (GenKI), a virtual knockout (KO) tool for gene function prediction using single-cell RNA sequencing (scRNA-seq) data in the absence of KO samples when only wild-type (WT) samples… read more here.

Keywords: cell; variational graph; gene knockout; single cell ... See more keywords

Txnip gene knockout ameliorated HFD-induced cardiomyopathy via regulating mitochondria dynamics and fatty acid oxidation.

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recommendations! Published in 2023 at "Journal of cardiovascular pharmacology"

DOI: 10.1097/fjc.0000000000001414

Abstract: ABSTRACT Epidemic of obesity accelerates the increase in the number of patients with obesity cardiomyopathy. Thioredoxin interacting protein (TXNIP) has been implicated in the pathogenesis of multiple cardiovascular diseases. However, its specific role in obesity… read more here.

Keywords: txnip; hfd; gene knockout; induced cardiomyopathy ... See more keywords