Creating Targeted Gene Knockouts in Brassica oleracea Using CRISPR/Cas9.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8991-1_12
Abstract: While public and political views on genetic modification (inserting "foreign" genes to elicit new traits) have resulted in limited exploitation of the technology in some parts of the world, the new era of genome editing… read more here.
Keywords: gene knockouts; creating targeted; brassica oleracea; gene ... See more keywords
CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e17-05-0288
Abstract: Conventional nonhomologous end joining–based gene knockouts can lead to the production of C-terminally truncated proteins with potentially residual or dominant negative functions. Combining CRISPR/Cas9 with gene traps targeting the first intron (CRISPR-Trap), however, completely prevents… read more here.
Keywords: gene knockouts; approach generation; clean approach; trap clean ... See more keywords