CasSABER for Programmable In Situ Visualization of Low and Nonrepetitive Gene Loci.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c04867
Abstract: Site-specific imaging of target genes using CRISPR probes is essential for understanding the molecular mechanisms of gene function and engineering tools to modulate its downstream pathways. Herein, we develop CRISPR/Cas9-mediated signal amplification by exchange reaction… read more here.
Keywords: nonrepetitive gene; cassaber programmable; low nonrepetitive; gene loci ... See more keywords
Imaging-Based In Situ Analysis of 5-Methylcytosine at Low Repetitive Single Gene Loci with Transcription-Activator-Like Effector Probes
Sign Up to like & getrecommendations! Published in 2023 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.2c00857
Abstract: Transcription-activator-like effectors (TALEs) are programmable DNA binding proteins that can be used for sequence-specific, imaging-based analysis of cellular 5-methylcytosine. However, this has so far been limited to highly repetitive satellite DNA. To expand this approach… read more here.
Keywords: transcription activator; gene loci; analysis; activator like ... See more keywords
Structural and spatial chromatin features at developmental gene loci in human pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-01679-x
Abstract: Higher-order chromatin organization controls transcriptional programs that govern cell properties and functions. In order for pluripotent stem cells (PSCs) to appropriately respond to differentiation signals, developmental gene loci should be structurally and spatially regulated to… read more here.
Keywords: developmental gene; chromatin; pluripotent stem; stem cells ... See more keywords
Kollector: transcript-informed, targeted de novo assembly of gene loci
Sign Up to like & getrecommendations! Published in 2017 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btx078
Abstract: Motivation: Despite considerable advancements in sequencing and computing technologies, de novo assembly of whole eukaryotic genomes is still a time‐consuming task that requires a significant amount of computational resources and expertise. A targeted assembly approach… read more here.
Keywords: novo assembly; kollector; gene; gene loci ... See more keywords
Autoregulation of ToxR and Its Regulatory Actions on Major Virulence Gene Loci in Vibrio parahaemolyticus
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2018.00291
Abstract: Vibrio parahaemolyticus, the leading causative agent of seafood-associated gastroenteritis, harbors two major virulence gene loci T3SS1 and Vp-PAI (T3SS2 and tdh2). ToxR is a virulence regulator of vibrios. Cell density-dependent transcriptional pattern of toxR and… read more here.
Keywords: major virulence; vibrio parahaemolyticus; virulence; virulence gene ... See more keywords
Identification of Marbling Gene Loci in Commercial Pigs in Canadian Herds
Sign Up to like & getrecommendations! Published in 2018 at "Agriculture"
DOI: 10.3390/agriculture8080122
Abstract: A genome-wide association study (GWAS) was performed on the intramuscular fat percentage in pork chops in commercially available swine in Canada. The Duroc, Iberian, Lacombe, Berkshire, and Pietrain breeds were crossed with Large White sows,… read more here.
Keywords: identification marbling; pork; loci commercial; marbling gene ... See more keywords