Efficient and error-free fluorescent gene tagging in human organoids without double-strand DNA cleavage
Sign Up to like & getrecommendations! Published in 2022 at "PLoS Biology"
DOI: 10.1371/journal.pbio.3001527
Abstract: CRISPR-associated nucleases are powerful tools for precise genome editing of model systems, including human organoids. Current methods describing fluorescent gene tagging in organoids rely on the generation of DNA double-strand breaks (DSBs) to stimulate homology-directed… read more here.
Keywords: gene tagging; fluorescent gene; human organoids; double strand ... See more keywords
Gene Tagging Strategies To Assess Protein Expression, Localization, and Function in Drosophila
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DOI: 10.1534/genetics.117.300392
Abstract: In the article by O. Kanca, H. Bellen, and F. Schnorrer (GENETICS 207: 389–412) entitled “Gene tagging strategies to assess protein expression, localization and function in Drosophila,” Figure 1 and Figure 8 were replaced as… read more here.
Keywords: protein expression; strategies assess; gene tagging; assess protein ... See more keywords
An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila
Sign Up to like & getrecommendations! Published in 2018 at "eLife"
DOI: 10.7554/elife.38709
Abstract: We generated two new genetic tools to efficiently tag genes in Drosophila. The first, Double Header (DH) utilizes intronic MiMIC/CRIMIC insertions to generate artificial exons for GFP mediated protein trapping or T2A-GAL4 gene trapping in… read more here.
Keywords: crispr; expanded toolkit; tagging based; toolkit gene ... See more keywords