Generation of Tfap2c‐T2A‐tdTomato knock‐in reporter rats via adeno‐associated virus‐mediated efficient gene targeting
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Reproduction and Development"
DOI: 10.1002/mrd.23562
Abstract: Gene editing in mammalian zygotes enables us to generate genetically modified animals rapidly and efficiently. In this study, we compare multiple gene targeting strategies in rat zygotes by generating a novel knock‐in reporter rat line… read more here.
Keywords: reporter; tfap2c t2a; gene targeting; tfap2c ... See more keywords
Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patens.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0621-6_8
Abstract: The moss Physcomitrella patens has become established as a model for investigating plant gene function due to the feasibility of gene targeting. The chemical composition of the P. patens cell wall is similar to that… read more here.
Keywords: physcomitrella patens; gene targeting; wall; cell wall ... See more keywords
CRISPR/Cas-Mediated In Planta Gene Targeting.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7003-2_1
Abstract: The recent emergence of the CRISPR/Cas system has boosted the possibilities for precise genome engineering approaches throughout all kingdoms of life. The most common application for plants is targeted mutagenesis, whereby a Cas9-mediated DNA double-strand… read more here.
Keywords: gene targeting; cas mediated; crispr cas; planta gene ... See more keywords
Deletion of ligD significantly improves gene targeting frequency in the lignocellulolytic filamentous fungus Penicillium oxalicum.
Sign Up to like & getrecommendations! Published in 2017 at "Fungal biology"
DOI: 10.1016/j.funbio.2017.04.005
Abstract: To improve the gene targeting frequency (GTF) in the lignocellulolytic filamentous fungus Penicillium oxalicum HP7-1, the non-homologous end-joining (NHEJ) gene ligD was deleted. The obtained PoligD deletion mutant ΔPoligD showed no apparent defect in cellulase… read more here.
Keywords: targeting frequency; lignocellulolytic filamentous; deletion; gene targeting ... See more keywords
A comparison of Agrobacterium-mediated transformation and protoplast-mediated transformation with CRISPR-Cas9 and bipartite gene targeting substrates, as effective gene targeting tools for Aspergillus carbonarius.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2017.01.015
Abstract: In recent years, versatile genetic tools have been developed and applied to a number of filamentous fungi of industrial importance. However, the existing techniques have limitations when it comes to achieve the desired genetic modifications,… read more here.
Keywords: agrobacterium; gene targeting; aspergillus carbonarius; gene ... See more keywords
Efficient gene targeting in soybean using Ochrobactrum haywardense-mediated delivery of a marker-free donor template
Sign Up to like & getrecommendations! Published in 2022 at "Plant Physiology"
DOI: 10.1093/plphys/kiac075
Abstract: Abstract Gene targeting (GT) for precise gene insertion or swap into pre-defined genomic location has been a bottleneck for expedited soybean precision breeding. We report a robust selectable marker-free GT system in soybean, one of… read more here.
Keywords: marker; gene targeting; ochrobactrum haywardense; gene ... See more keywords
Introduction of a Recombineering Oligonucleotide and a CRISPR-Cas9 Gene-Targeting Plasmid into Staphylococcus ureus for Generating a Gene-Deletion Strain.
Sign Up to like & getrecommendations! Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107921
Abstract: Gene deletions can be generated in Staphylococcus aureus using recombineering in combination with a CRISPR-Cas9 counterselection approach. The method involves first designing the recombineering oligonucleotides and generating the relevant plasmids, and then introducing these elements… read more here.
Keywords: crispr cas9; deletion; gene targeting; strain ... See more keywords
Identification of Editable Sites, Spacer Oligonucleotide Design, and Generation of the Gene-Targeting CRISPR-nCas9 Plasmid for Gene Disruption in Staphylococcus aureus Using the CRISPR-nCas9 and Cytidine Deaminase System.
Sign Up to like & getrecommendations! Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107924
Abstract: Methods for gene disruption are essential for functional genomics, and there are multiple approaches for altering gene function in bacteria. One of these methods involves introducing a premature stop codon in a gene of interest,… read more here.
Keywords: ncas9 cytidine; gene targeting; cytidine deaminase; gene ... See more keywords
Introduction of a CRISPR-nCas9 Gene-Targeting Plasmid into Staphylococcus aureus for Gene Disruption.
Sign Up to like & getrecommendations! Published in 2023 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot107925
Abstract: Methods for gene disruption are essential for functional genomics, and there are multiple approaches for altering gene function in bacteria. One of these methods involves introducing a premature stop codon in a gene of interest,… read more here.
Keywords: gene disruption; introduction; gene targeting; gene ... See more keywords
Gene targeting as a therapeutic avenue in diseases mediated by the complement alternative pathway
Sign Up to like & getrecommendations! Published in 2022 at "Immunological Reviews"
DOI: 10.1111/imr.13149
Abstract: The complement alternative pathway (AP) is implicated in numerous diseases affecting many organs, ranging from the rare hematological disease paroxysmal nocturnal hemoglobinuria (PNH), to the common blinding disease age‐related macular degeneration (AMD). Critically, the AP… read more here.
Keywords: targeting therapeutic; alternative pathway; gene targeting; complement alternative ... See more keywords
A New EBS2b-IBS2b Base Paring (A−8/T−8) Improved the Gene-Targeting Efficiency of Thermotargetron in Escherichia coli
Sign Up to like & getrecommendations! Published in 2023 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.03159-22
Abstract: The randomized base pairing in the interval of IBS2 and IBS1 of Tel3c/4c intron (−8 and −7 sites) in Thermotargetron (TMT) results in a low success rate and gene-targeting efficiency in bacteria. In the present… read more here.
Keywords: intron; gene targeting; gene; base ... See more keywords