Ancient endosymbiont‐mediated transmission of a selfish gene provides a model for overcoming barriers to gene transfer into animal mitochondrial genomes
Sign Up to like & getrecommendations! Published in 2022 at "BioEssays"
DOI: 10.1002/bies.202200190
Abstract: In contrast to bilaterian animals, non‐bilaterian mitochondrial genomes contain atypical genes, often attributed to horizontal gene transfer (HGT) as an ad hoc explanation. Although prevalent in plants, HGT into animal mitochondrial genomes is rare, lacking… read more here.
Keywords: provides model; ancient endosymbiont; mitochondrial genomes; gene transfer ... See more keywords
Advancements in the design and scalable production of viral gene transfer vectors
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26461
Abstract: The last 10 years have seen a rapid expansion in the use of viral gene transfer vectors, with approved therapies and late stage clinical trials underway for the treatment of genetic disorders, and multiple forms… read more here.
Keywords: gene transfer; transfer vectors; production; viral gene ... See more keywords
MicroRNA‐based recombinant AAV vector assembly improves efficiency of suicide gene transfer in a murine model of lymphoma
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Medicine"
DOI: 10.1002/cam4.2935
Abstract: Recent success in clinical trials with recombinant Adeno‐associated virus (AAV)‐based gene therapy has redirected efforts in optimizing AAV assembly and production, to improve its potency. We reasoned that inclusion of a small RNA during vector… read more here.
Keywords: vector; suicide gene; vector assembly; gene transfer ... See more keywords
Efficacy of hydrodynamic interleukin 10 gene transfer in human liver segments with interest in transplantation
Sign Up to like & getrecommendations! Published in 2017 at "Liver Transplantation"
DOI: 10.1002/lt.24667
Abstract: Different diseases lead, during their advanced stages, to chronic or acute liver failure, whose unique treatment consists in organ transplantation. The success of intervention is limited by host immune response and graft rejection. The use… read more here.
Keywords: transfer human; liver; transplantation; gene transfer ... See more keywords
Extracellular vesicles: nature's nanoparticles for improving gene transfer with adeno-associated virus vectors.
Sign Up to like & getrecommendations! Published in 2018 at "Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology"
DOI: 10.1002/wnan.1488
Abstract: Gene therapy, the ability to treat a disease at the level of nucleic acid, has journeyed from science fiction, to hard lessons learned from early clinical trials, to improved technologies with efficacy in patients for… read more here.
Keywords: associated virus; aav vectors; adeno associated; gene transfer ... See more keywords
In Vivo Eosinophil Expansion Using Gene Transfer by Electroporation.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1095-4_11
Abstract: Eosinophils are differentiated in the bone marrow and transit through the blood circulation to home into tissues primarily under the regulation of IL-5. Because the number of eosinophils in the peripheral blood is relatively low… read more here.
Keywords: eosinophil expansion; transfer electroporation; expansion using; using gene ... See more keywords
Gene Transfer to Mouse Kidney In Vivo.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9065-8_14
Abstract: Genetic nephropathies represent a challenging class of disorders to be treated by gene therapy. This is primarily due to the filtering properties of the kidney itself, which does not allow the vehicle carrying the transgene… read more here.
Keywords: transfer mouse; kidney vivo; gene transfer; gene ... See more keywords
Methods for the Analysis and Characterization of Defense Mechanisms Against Horizontal Gene Transfer: CRISPR Systems.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9877-7_17
Abstract: CRISPR-Cas systems provide RNA-guided adaptive immunity to the majority of archaea and many bacteria. They are able to capture pieces of invading genetic elements in the form of novel spacers in an array of repeats.… read more here.
Keywords: gene transfer; crispr cas; cas systems; horizontal gene ... See more keywords
Indirect identification of horizontal gene transfer
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Mathematical Biology"
DOI: 10.1007/s00285-021-01631-0
Abstract: Several implicit methods to infer horizontal gene transfer (HGT) focus on pairs of genes that have diverged only after the divergence of the two species in which the genes reside. This situation defines the edge… read more here.
Keywords: hgt; graph; transfer; gene transfer ... See more keywords
Patient Perspectives on Gene Transfer Therapy for Sickle Cell Disease
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DOI: 10.1007/s12325-017-0587-7
Abstract: IntroductionSickle cell disease (SCD) is a chronic genetic disease with high morbidity and early mortality; it affects nearly 100,000 individuals in the USA. Bone marrow transplantation, the only curative treatment, is available to less than… read more here.
Keywords: transfer therapy; patient perspectives; disease; cell disease ... See more keywords
Aminoclay as a highly effective cationic vehicle for enhancing adenovirus-mediated gene transfer through nanobiohybrid complex formation.
Sign Up to like & getrecommendations! Published in 2017 at "Acta biomaterialia"
DOI: 10.1016/j.actbio.2016.11.045
Abstract: Electrostatic complexation of adenovirus (Ad) with cationic lipids or polymers has been shown to be an effective means for overcoming the limitations of adenoviral vectors and enhancing gene-transfer efficacy. However, such complexation causes cytotoxicity, limiting… read more here.
Keywords: mediated gene; complexation; gene transfer; gene ... See more keywords