A general platform for efficient extracellular expression and purification of Fab from Escherichia coli
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DOI: 10.1007/s00253-019-09745-8
Abstract: Antigen-binding fragments (Fabs) are an important part of monoclonal antibody (mAb) therapeutics and can be cost-effectively produced using an Escherichia coli (E. coli) expression system. However, Fabs tend to form undesirable aggregates when expressed in… read more here.
Keywords: general platform; escherichia coli; fab fragments; fab ... See more keywords
A general platform for antibody purification utilizing engineered-micelles
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DOI: 10.1080/19420862.2019.1565749
Abstract: ABSTRACT We introduce a new concept and potentially general platform for antibody (Ab) purification that does not rely on chromatography or specific ligands (e.g., Protein A); rather, it makes use of detergent aggregates capable of… read more here.
Keywords: platform antibody; antibody purification; engineered micelles; general platform ... See more keywords