Generation of deletions and precise point mutations in Dictyostelium discoideum using the CRISPR nickase
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DOI: 10.1371/journal.pone.0224128
Abstract: The CRISPR/Cas9 system enables targeted genome modifications across a range of eukaryotes. Although we have reported that transient introduction of all-in-one vectors that express both Cas9 and sgRNAs can efficiently induce multiple gene knockouts in… read more here.
Keywords: point mutations; dictyostelium discoideum; precise point; discoideum ... See more keywords