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   Articles with "genes qpcr" as a keyword



Selecting reference genes in RT-qPCR based on equivalence tests: a network based approach

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recommendations! Published in 2019 at "Scientific Reports"

DOI: 10.1038/s41598-019-52217-2

Abstract: Because quantitative reverse transcription PCR (RT-qPCR) gene expression data are compositional, amounts of quantified RNAs must be normalized using reference genes. However, the two most used methods to select reference genes (NormFinder and geNorm) ignore… read more here.

Keywords: selecting reference; reference; equivalence tests; reference genes ... See more keywords

Reference Genes for qPCR-Based miRNA Expression Profiling in 14 Human Tissues

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recommendations! Published in 2022 at "Medical Principles and Practice"

DOI: 10.1159/000524283

Abstract: MicroRNAs (miRNAs) are promising biomarkers for the diagnosis and prognosis of various diseases. Quantitative PCR is the most frequently used method of measuring expression levels of miRNA. However, the lack of validated reference genes represents… read more here.

Keywords: expression; genes qpcr; qpcr based; reference genes ... See more keywords

Appropriate Reference Genes for RT-qPCR Normalization in Various Organs of Anemone flaccida Fr. Schmidt at Different Growing Stages

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recommendations! Published in 2021 at "Genes"

DOI: 10.3390/genes12030459

Abstract: Anemone flaccida Fr. Schmidt is a traditional medicinal herb in southwestern China and has multiple pharmacological effects on bruise injuries and rheumatoid arthritis (RA). A new drug with a good curative effect on RA has… read more here.

Keywords: anemone flaccida; flaccida schmidt; reference; various organs ... See more keywords

Selection and Validation of Reference Genes for RT-qPCR in Protonemal Tissue of the Desiccation-Tolerant Moss Pseudocrossidium replicatum Under Multiple Abiotic Stress Conditions

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recommendations! Published in 2025 at "Plants"

DOI: 10.3390/plants14121752

Abstract: Plant abiotic stresses are the main cause of significant crop losses worldwide. The moss Pseudocrossidium replicatum is highly tolerant to different types of abiotic stress, such as desiccation. Our group is interested in identifying and… read more here.

Keywords: reference genes; desiccation; abiotic stress; genes qpcr ... See more keywords