Selecting reference genes in RT-qPCR based on equivalence tests: a network based approach
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DOI: 10.1038/s41598-019-52217-2
Abstract: Because quantitative reverse transcription PCR (RT-qPCR) gene expression data are compositional, amounts of quantified RNAs must be normalized using reference genes. However, the two most used methods to select reference genes (NormFinder and geNorm) ignore… read more here.
Keywords: selecting reference; reference; equivalence tests; reference genes ... See more keywords
Reference Genes for qPCR-Based miRNA Expression Profiling in 14 Human Tissues
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DOI: 10.1159/000524283
Abstract: MicroRNAs (miRNAs) are promising biomarkers for the diagnosis and prognosis of various diseases. Quantitative PCR is the most frequently used method of measuring expression levels of miRNA. However, the lack of validated reference genes represents… read more here.
Keywords: expression; genes qpcr; qpcr based; reference genes ... See more keywords
Appropriate Reference Genes for RT-qPCR Normalization in Various Organs of Anemone flaccida Fr. Schmidt at Different Growing Stages
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DOI: 10.3390/genes12030459
Abstract: Anemone flaccida Fr. Schmidt is a traditional medicinal herb in southwestern China and has multiple pharmacological effects on bruise injuries and rheumatoid arthritis (RA). A new drug with a good curative effect on RA has… read more here.
Keywords: anemone flaccida; flaccida schmidt; reference; various organs ... See more keywords