In vitro determination of essential genes required by Streptococcus uberis to grow in a complex biological media relating to intramammary infection
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DOI: 10.1099/mgen.0.001425
Abstract: Abstract Streptococcus uberis is a leading cause of bovine mastitis, a disease of significant economic impact to the dairy industry. Understanding the genetic factors essential for bacterial survival in milk and during intramammary infection is… read more here.
Keywords: streptococcus uberis; uberis grow; intramammary infection; genes required ... See more keywords
Identification of Genes Required for Swarming Motility in Bacillus subtilis Using Transposon Mutagenesis and High-Throughput Sequencing (TnSeq)
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DOI: 10.1128/jb.00089-22
Abstract: In TnSeq, transposons are randomly inserted throughout the chromosome at a population level, but insertions that disrupt genes of essential function cause strains that carry them to fall out of the population and appear underrepresented… read more here.
Keywords: genes required; transposon mutagenesis; bacillus subtilis; swarming motility ... See more keywords
Identification and Characterization of Genes Required for 5-Hydroxyuridine Synthesis in Bacillus subtilis and Escherichia coli tRNA
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DOI: 10.1128/jb.00433-19
Abstract: Modified nucleotides in tRNA serve to optimize both its structure and function for accurate translation of the genetic code. The biosynthesis of these modifications has been fertile ground for uncovering unique biochemistry and metabolism in… read more here.
Keywords: biochemistry; bacillus subtilis; escherichia coli; biosynthesis ... See more keywords