Synthetic Genetic Circuits Enabled in Komagataella phaffii Through T7 RNAP/CRISPRa System
Sign Up to like & getrecommendations! Published in 2025 at "Biotechnology Journal"
DOI: 10.1002/biot.70036
Abstract: The CRISPR activation (CRISPRa) transcriptional system has become a powerful synthetic biology tool for the regulation of endogenous gene expression, allowing for precise fine‐tuning of target genes through the simple modification of sgRNA sequences. In… read more here.
Keywords: system; genetic circuits; rnap; komagataella phaffii ... See more keywords
Cellular Biocatalysts Using Synthetic Genetic Circuits for Prolonged and Durable Enzymatic Activity
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201800767
Abstract: Cellular biocatalysts hold great promise for the synthesis of difficult to achieve compounds, such as complex active molecules. Whole‐cell biocatalysts can be programmed through genetic circuits to be more efficient, but they suffer from low… read more here.
Keywords: genetic circuits; whole cell; biofilm; cellular biocatalysts ... See more keywords
Encoding Genetic Circuits with DNA Barcodes Paves the Way for Machine Learning-Assisted Metabolite Biosensor Response Curve Profiling in Yeast.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00595
Abstract: Genetically encoded biosensors are valuable tools used in the precise engineering of metabolism. Although a large number of biosensors have been developed, the fine-tuning of their dose-response curves, which promotes the applications of biosensors in… read more here.
Keywords: biosensor; biosensor response; genetic circuits; machine learning ... See more keywords
Insulated transcriptional elements enable precise design of genetic circuits
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/s41467-017-00063-z
Abstract: Rational engineering of biological systems is often complicated by the complex but unwanted interactions between cellular components at multiple levels. Here we address this issue at the level of prokaryotic transcription by insulating minimal promoters… read more here.
Keywords: engineering; transcription; genetic circuits; insulated transcriptional ... See more keywords
Engineering wetware and software for the predictive design of compressed genetic circuits for higher-state decision-making
Sign Up to like & getrecommendations! Published in 2025 at "Nature Communications"
DOI: 10.1038/s41467-025-64457-0
Abstract: Synthetic genetic circuits enable the reprogramming of cells, advancing the study and application of biology with greater precision. However, quantitative circuit design is hampered by the limited modularity of biological parts. As circuit complexity increases,… read more here.
Keywords: genetic circuits; wetware; software; higher state ... See more keywords
Pyruvate-responsive genetic circuits for dynamic control of central metabolism
Sign Up to like & getrecommendations! Published in 2020 at "Nature Chemical Biology"
DOI: 10.1038/s41589-020-0637-3
Abstract: Dynamic regulation is a promising strategy for fine-tuning metabolic fluxes in microbial cell factories. However, few of these synthetic regulatory systems have been developed for central carbon metabolites. Here we created a set of programmable… read more here.
Keywords: pyruvate responsive; genetic circuits; central metabolism; responsive genetic ... See more keywords
Dynamics and bifurcations in genetic circuits with fibration symmetries
Sign Up to like & getrecommendations! Published in 2024 at "Journal of the Royal Society Interface"
DOI: 10.1098/rsif.2024.0386
Abstract: Circuit building blocks of gene regulatory networks (GRN) have been identified through the fibration symmetries of the underlying biological graph. Here, we analyse analytically six of these circuits that occur as functional and synchronous building… read more here.
Keywords: bifurcations genetic; genetic circuits; fibration symmetries; fibration ... See more keywords
Optimization of Transcription Factor Genetic Circuits
Sign Up to like & getrecommendations! Published in 2022 at "Biology"
DOI: 10.1101/2022.07.05.498863
Abstract: Transcription factors (TFs) affect the expression of mRNAs. In essence, the TFs form a large computation network that controls many aspects of cellular function. This article introduces a computational method to optimize TF networks. The… read more here.
Keywords: optimization transcription; factor genetic; transcription; genetic circuits ... See more keywords
Chimeric MerR-Family Regulators and Logic Elements for the Design of Metal Sensitive Genetic Circuits in Bacillus subtilis
Sign Up to like & getrecommendations! Published in 2022 at "ACS Synthetic Biology"
DOI: 10.1101/2022.10.13.512145
Abstract: Whole-cell biosensors are emerging as promising tools for monitoring environmental pollutants such as heavy metals. These sensors constitute a genetic circuit comprising a sensing module and an output module, such that a detectable signal is… read more here.
Keywords: merr family; genetic circuits; design; metal ... See more keywords
Parity-Check Coding Based on Genetic Circuits for Engineered Molecular Communication Between Biological Cells
Sign Up to like & getrecommendations! Published in 2018 at "IEEE Transactions on Communications"
DOI: 10.1109/tcomm.2018.2859308
Abstract: Synthetic biology, through genetic circuit engineering in biological cells, is paving the way toward the realization of programmable man-made living devices, able to naturally operate within normally less accessible domains, i.e., the biological and the… read more here.
Keywords: biological cells; genetic circuits; molecular communication; parity check ... See more keywords
Convolutional Codec Implemented by Genetic Circuits for Molecular Communication
Sign Up to like & getrecommendations! Published in 2022 at "IEEE Transactions on NanoBioscience"
DOI: 10.1109/tnb.2022.3156621
Abstract: Molecular communication (MC), which transmits information through molecules, has emerged as a promising technique to enable communication links between nanomachines. To establish information transmission using molecules, synthetic biology through genetic circuits techniques can be utilized… read more here.
Keywords: codec implemented; communication; genetic circuits; molecular communication ... See more keywords