Efficient and precise control of gene expression in Geobacter sulfurreducens through new genetic elements and tools for pollutant conversion.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.28433
Abstract: Geobacter species, exhibiting exceptional extracellular electron transfer aptitude, hold great potential for applications in pollution remediation, bioenergy production, and natural elemental cycles. Nonetheless, a scarcity of well-characterized genetic elements and gene expression tools constrains the… read more here.
Keywords: genetic elements; geobacter; gene expression; expression geobacter ... See more keywords
Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate Assembly.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0868-5_11
Abstract: Budding yeast Saccharomyces cerevisiae has become a model eukaryotic microorganism for targeted genomic manipulation due to its efficient homologous recombination. A few genomic loci, including rDNA, Delta, and Ty1, can be utilized to introduce variable… read more here.
Keywords: copy; gate assembly; genetic elements; complex genetic ... See more keywords
intI1 Type Mobile Genetic Elements Co-selected Antibiotic-Resistant Genes in Untreated Hospital Wastewaters
Sign Up to like & getrecommendations! Published in 2021 at "Bulletin of Environmental Contamination and Toxicology"
DOI: 10.1007/s00128-020-03098-z
Abstract: Dissemination of antibiotic-resistant genes (ARGs) from hospital wastewaters (HWWs) is facilitated by the horizontal gene transfer (HGT) and involves association of ARGs with mobile genetic elements (MGEs). In our previous study, HWWs were found to… read more here.
Keywords: mobile genetic; antibiotic resistant; copy; hospital wastewaters ... See more keywords
Can mobile genetic elements rescue genes from extinction
Sign Up to like & getrecommendations! Published in 2020 at "Current Genetics"
DOI: 10.1007/s00294-020-01104-9
Abstract: Bacteria and other prokaryotes evolve primarily through rapid changes in their gene content by quickly losing and gaining genes whenever an ecological opportunity emerges. As gene loss and horizontal gene transfer (HGT) appear to be… read more here.
Keywords: genetic elements; mobile genetic; gene; rescue genes ... See more keywords
Solid-state anaerobic digestion facilitates the removal of antibiotic resistance genes and mobile genetic elements from cattle manure.
Sign Up to like & getrecommendations! Published in 2019 at "Bioresource technology"
DOI: 10.1016/j.biortech.2018.09.013
Abstract: Livestock manure is a hotspot for antibiotic resistance genes (ARGs). Solid-state anaerobic digestion (SAD) is a high efficiency livestock manure treatment, but the changes in ARGs and microbial communities during SAD are unknown. Therefore, thermophilic… read more here.
Keywords: mobile genetic; antibiotic resistance; genetic elements; anaerobic digestion ... See more keywords
A method to assemble DNA fragments mimicking junctions of transgenic elements: Application to the AquAdvantage salmon
Sign Up to like & getrecommendations! Published in 2017 at "Food Control"
DOI: 10.1016/j.foodcont.2017.06.037
Abstract: Abstract The detection of transgenic animals in food and environmental samples will require the development of reliable screening assays. The identification of junction regions between genetic elements is the unequivocal way to prove the presence… read more here.
Keywords: method assemble; dna; method; genetic elements ... See more keywords
Hyperthermophilic Composting Accelerates the Removal of Antibiotic Resistance Genes and Mobile Genetic Elements in Sewage Sludge.
Sign Up to like & getrecommendations! Published in 2018 at "Environmental science & technology"
DOI: 10.1021/acs.est.7b04483
Abstract: Composting is an efficient way to convert organic waste into fertilizers. However, waste materials often contain large amounts of antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) that can reduce the efficacy of antibiotic… read more here.
Keywords: hyperthermophilic composting; mobile genetic; args mges; antibiotic resistance ... See more keywords
Identification of misexpressed genetic elements in hybrids between Drosophila-related species
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep40618
Abstract: Crosses between close species can lead to genomic disorders, often considered to be the cause of hybrid incompatibility, one of the initial steps in the speciation process. How these incompatibilities are established and what are… read more here.
Keywords: parental lines; elements hybrids; hybrids drosophila; genetic elements ... See more keywords
It is unclear how important CRISPR-Cas systems are for protecting natural populations of bacteria against infections by mobile genetic elements
Sign Up to like & getrecommendations! Published in 2020 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1915966117
Abstract: Articles on CRISPR commonly open with some variant of the phrase “these short palindromic repeats and their associated endonucleases (Cas) are an adaptive immune system that exists to protect bacteria and archaea from viruses and… read more here.
Keywords: genetic elements; crispr cas; cas systems; mobile genetic ... See more keywords
Coevolution of reproducers and replicators at the origin of life and the conditions for the origin of genomes
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2301522120
Abstract: There are two fundamentally distinct but inextricably linked types of biological evolutionary units, reproducers and replicators. Reproducers are cells and organelles that reproduce via various forms of division and maintain the physical continuity of compartments… read more here.
Keywords: reproducers replicators; origin life; genetic elements; division ... See more keywords
Regulation of RNA polymerase III transcription during transformation of human IMR90 fibroblasts with defined genetic elements
Sign Up to like & getrecommendations! Published in 2018 at "Cell Cycle"
DOI: 10.1080/15384101.2017.1405881
Abstract: ABSTRACT RNA polymerase (Pol) III transcribes small untranslated RNAs that are essential for cellular homeostasis and growth. Its activity is regulated by inactivation of tumor suppressor proteins and overexpression of the oncogene c-MYC, but the… read more here.
Keywords: genetic elements; rna polymerase; transformation; pol iii ... See more keywords