LAUSR

Genetic Encoding of Phosphorylated Amino Acids into Proteins.

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0 Published in 2024 at "Chemical reviews"

DOI: 10.1021/acs.chemrev.4c00110

Abstract: Reversible phosphorylation is a fundamental mechanism for controlling protein function. Despite the critical roles phosphorylated proteins play in physiology and disease, our ability to study individual phospho-proteoforms has been hindered by a lack of versatile… read more here.

Keywords: encoding phosphorylated; amino acids; acids proteins; phosphorylated amino ... See more keywords

Genetic Encoding of Three Distinct Noncanonical Amino Acids Using Reprogrammed Initiator and Nonsense Codons.

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0 Published in 2021 at "ACS chemical biology"

DOI: 10.1021/acschembio.1c00120

Abstract: We recently described an orthogonal initiator tRNA (itRNATy2) that can initiate protein synthesis with noncanonical amino acids (ncAAs) in response to the UAG nonsense codon. Here, we report that a mutant of itRNATy2 (itRNATy2AUA) can… read more here.

Keywords: noncanonical amino; initiator; amino acids; genetic encoding ... See more keywords

Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells.

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1 Published in 2022 at "Chemical communications"

DOI: 10.1039/d2cc01578a

Abstract: Protein tyrosine phosphorylation plays a critical role in signal transduction. We report the genetic incorporation of a phosphotyrosine (pTyr) analog, p-carboxymethyl-L-phenylalanine (CMF), into proteins in mammalian cells. This nonhydrolyzable pTyr analog can facilitate biological studies… read more here.

Keywords: nonhydrolyzable phosphotyrosine; analog; encoding nonhydrolyzable; mammalian cells ... See more keywords

Genetic encoding of ε-N-L-lactyllysine for detecting delactylase activity in living cells.

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1 Published in 2022 at "Chemical communications"

DOI: 10.1039/d2cc02643k

Abstract: Lysine ε-N-L-lactylation is a newly discovered post-translational modification. Herein we present the genetic encoding of ε-N-L-lactyllysine in bacterial and mammalian cells, allowing the preparation of site-specifically ε-N-L-lactylated recombinant proteins and the construction of fluorescent and… read more here.

Keywords: living cells; detecting delactylase; genetic encoding; lactyllysine detecting ... See more keywords

Photo-regulated genetic encoding of dibenzo[c,g][1,2]diazocine on proteins via configuration switching.

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2 Published in 2023 at "Chemical communications"

DOI: 10.1039/d2cc06738b

Abstract: We report two evolved Methanosarcina mazei pyrrolysine tRNA synthetases to genetically incorporate the isomers of dibenzo[c,g][1,2]diazocine-alanine (DBDAA) into proteins either in the dark or under regulation of 405 nm photo-stimulation. The genetic-encoded DBDAA realizes photo-tuning… read more here.

Keywords: photo; regulated genetic; encoding dibenzo; dibenzo diazocine ... See more keywords