X‐ray‐Guided In Situ Genetic Engineering of Macrophages for Sustained Cancer Immunotherapy
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Materials"
DOI: 10.1002/adma.202208059
Abstract: Effective repolarization of macrophages has emerged as a promising approach for anticancer therapy. However, there are very few studies on the effect of reprogramming macrophages from M2 phenotype to M1 phenotype without reconversion while maintaining… read more here.
Keywords: genetic engineering; engineering macrophages; ray guided; situ genetic ... See more keywords
Bio‐engineering of microalgae: Challenges and future prospects toward industrial and environmental applications
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.202100417
Abstract: Microalgae are complex metabolic machineries whose potential has been continuously tapped for wide range of applications. Their suboptimal biomass yield and bioactives production remain the mainstay for their commercialization. To surpass the limitations, genetic engineering… read more here.
Keywords: genetic engineering; bio engineering; microalgae challenges; microalgae ... See more keywords
Efficient genetic engineering of murine cochlear organoids
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Tissue Engineering and Regenerative Medicine"
DOI: 10.1002/term.3298
Abstract: Organoid culture is a popular model to study gene function as the easy manipulating and time saving compared with in vivo experiments. This is widely used in auditory system for studying supporting cells (SCs) or… read more here.
Keywords: genetic engineering; engineering murine; efficient genetic; murine cochlear ... See more keywords
Development of a dual-antimicrobial counterselection method for markerless genetic engineering of bacterial genomes
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-9565-5
Abstract: Markerless genetic engineering of bacterial genomes is commonly performed by two-step homologous recombination methods using vectors carrying flanking regions of the target gene for site-specific vector integration and counterselection markers to provide positive selection pressure… read more here.
Keywords: markerless genetic; method; selection; counterselection ... See more keywords
CRISPR-Cas9-assisted native end-joining editing offers a simple strategy for efficient genetic engineering in Escherichia coli
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-10104-w
Abstract: Unlike eukaryotes, prokaryotes are less proficient in homologous recombination (HR) and non-homologous end-joining (NHEJ). All existing genomic editing methods for Escherichia coli (E. coli) rely on exogenous HR or NHEJ systems to repair DNA double-strand… read more here.
Keywords: end joining; end; native end; genetic engineering ... See more keywords
Current achievements and future prospects of genetic engineering in Indian mustard (Brassica juncea L. Czern & Coss.).
Sign Up to like & getrecommendations! Published in 2020 at "Planta"
DOI: 10.1007/s00425-020-03461-8
Abstract: MAIN CONCLUSION Transgenic technology in Indian mustard has expedited crop improvement programs. Further, there is a need to optimize gene editing protocols and find out the suitable target genes to harvest the benefits of gene… read more here.
Keywords: indian mustard; mustard; genetic engineering; gene ... See more keywords
Genetic engineering of crops for insect resistance: An overview
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Biosciences"
DOI: 10.1007/s12038-020-00081-y
Abstract: Phytophagous insect incidence is a serious threat for reduction of crop productivity globally. There is an estimation of one fourth of crop is being destroyed by insects annually. Indeed, the development of insect-resistant crops is… read more here.
Keywords: insects; resistant crops; insect resistant; resistance ... See more keywords
An update and perspectives on the use of promoters in plant genetic engineering
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Biosciences"
DOI: 10.1007/s12038-020-00087-6
Abstract: Genetically engineered plants have varied applications in agriculture for enhancing the values of food and feed. Genetic engineering aims to introduce selected genetic regions with desirable traits into target plants for both spatial and temporal… read more here.
Keywords: promoters plant; engineering; use promoters; update perspectives ... See more keywords
Application of Genetic Engineering in Biotherapeutics Development
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Pharmaceutical Innovation"
DOI: 10.1007/s12247-019-09411-6
Abstract: Purpose Advancements in genetic engineering have profoundly impacted the biotherapeutic industry and shaped many of the methods and strategies used today for both bioproduction and therapeutic design. Improvements in production yield and quality have been… read more here.
Keywords: application genetic; engineering; bioproduction; gene therapy ... See more keywords
Genetic engineering strategies for biotic and abiotic stress tolerance and quality enhancement in horticultural crops: a comprehensive review
Sign Up to like & getrecommendations! Published in 2017 at "3 Biotech"
DOI: 10.1007/s13205-017-0870-y
Abstract: Genetic engineering technique offers myriads of applications in improvement of horticultural crops for biotic and abiotic stress tolerance, and produce quality enhancement. During last two decades, a large number of transgenic horticultural crops has been… read more here.
Keywords: stress tolerance; horticultural crops; stress; genetic engineering ... See more keywords
A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering
Sign Up to like & getrecommendations! Published in 2019 at "3 Biotech"
DOI: 10.1007/s13205-019-1861-y
Abstract: Antifreeze proteins (AFPs) confer the ability to survive at subzero temperatures and are found in many different organisms, including fish, plants, and insects. They prevent the formation of ice crystals by non-colligative adsorption to the… read more here.
Keywords: insects; antifreeze proteins; metabolic genetic; cryopreservation ... See more keywords