Genetic manipulation and targeted protein degradation in mammalian systems: practical considerations, tips and tricks for discovery research.
Sign Up to like & getrecommendations! Published in 2023 at "FEBS open bio"
DOI: 10.1002/2211-5463.13581
Abstract: Gaining a mechanistic understanding of the molecular pathways underpinning cellular and organismal physiology invariably relies on perturbation of an experimental system to infer causality. This can be achieved either by genetic manipulation or by pharmacological… read more here.
Keywords: mammalian systems; genetic manipulation; targeted protein; manipulation ... See more keywords
Genetic Manipulation of Group A Streptococcus-Gene Deletion by Allelic Replacement.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0467-0_5
Abstract: Genetic manipulation of Streptococcus pyogenes (Group A Streptococcus, GAS) has historically been a challenging process, with considerable variation in efficiency between different strains. Here, we outline an optimized, rapid method for creating markerless isogenic mutations… read more here.
Keywords: manipulation group; group streptococcus; streptococcus gene; genetic manipulation ... See more keywords
Effects of genetic manipulation of the activity of photorespiration on the redox state of photosystem I and its robustness against excess light stress under CO2-limited conditions in rice
Sign Up to like & getrecommendations! Published in 2018 at "Photosynthesis Research"
DOI: 10.1007/s11120-018-0515-y
Abstract: Under CO2-limited conditions such as during stomatal closure, photorespiration is suggested to act as a sink for excess light energy and protect photosystem I (PSI) by oxidizing its reaction center chlorophyll P700. In this study,… read more here.
Keywords: genetic manipulation; light stress; photorespiration; excess light ... See more keywords
Genetic manipulation of phages for therapy using BRED.
Sign Up to like & getrecommendations! Published in 2020 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2020.09.005
Abstract: The alarming increase in antibiotic resistance has placed the focus on phages as an alternative antimicrobial therapy. Recently, the first patient treatment using engineered phages to combat a mycobacterial infection was successfully performed; genetic modifications… read more here.
Keywords: genetic manipulation; therapy using; using bred; manipulation phages ... See more keywords
Methods for the genetic manipulation of marine bacteria
Sign Up to like & getrecommendations! Published in 2018 at "Electronic Journal of Biotechnology"
DOI: 10.1016/j.ejbt.2018.03.003
Abstract: Genetic manipulation of bacteria is a procedure necessary to obtain new strains that express peculiar and defined genetic determinants or to introduce genetic variants responsible for phenotypic modifications. This procedure can be applied to explore… read more here.
Keywords: marine bacteria; genetic manipulation; methods genetic; manipulation marine ... See more keywords
Unmarked genetic manipulation in Bacillus subtilis by natural co-transformation.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2018.08.001
Abstract: Bacillus subtilis is well known as both a model organism and as a microbial cell factory. Simple and scarless gene modification is a desirable tool for basic research and industrial applications of B. subtilis. It… read more here.
Keywords: genetic manipulation; method; transformation; unmarked genetic ... See more keywords
Improving acarbose production and eliminating the by-product component C with an efficient genetic manipulation system of Actinoplanes sp. SE50/110
Sign Up to like & getrecommendations! Published in 2017 at "Synthetic and Systems Biotechnology"
DOI: 10.1016/j.synbio.2017.11.005
Abstract: The α-glucosidase inhibitor acarbose is commercially produced by Actinoplanes sp. and used as a potent drug in the treatment of type-2 diabetes. In order to improve the yield of acarbose, an efficient genetic manipulation system… read more here.
Keywords: efficient genetic; system; genetic manipulation; component ... See more keywords
Noninvasive optical activation of Flp recombinase for genetic manipulation in deep mouse brain regions
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-018-08282-8
Abstract: Spatiotemporal control of gene expression or labeling is a valuable strategy for identifying functions of genes within complex neural circuits. Here, we develop a highly light-sensitive and efficient photoactivatable Flp recombinase (PA-Flp) that is suitable… read more here.
Keywords: flp recombinase; flp; mouse; genetic manipulation ... See more keywords
SPARC enables genetic manipulation of precise proportions of cells.
Sign Up to like & getrecommendations! Published in 2020 at "Nature neuroscience"
DOI: 10.1038/s41593-020-0668-9
Abstract: Many experimental approaches rely on controlling gene expression in select subsets of cells within an individual animal. However, reproducibly targeting transgene expression to specific fractions of a genetically defined cell type is challenging. We developed… read more here.
Keywords: genetic manipulation; precise proportions; enables genetic; manipulation precise ... See more keywords
Expansion, in vivo–ex vivo cycling, and genetic manipulation of primary human hepatocytes
Sign Up to like & getrecommendations! Published in 2020 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.1919035117
Abstract: Significance The ability to study human liver disease is limited by available hepatocyte models. Primary human hepatocytes (PHH) and xenograft models suffer from limited availability, donor-to-donor variability, and high cost. Here we report two transformative… read more here.
Keywords: genetic manipulation; vivo; disease; liver ... See more keywords
Genetic Manipulation by Zinc-Finger Nucleases in Rat-Induced Pluripotent Stem Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Cellular Reprogramming"
DOI: 10.1089/cell.2016.0028
Abstract: Abstract Induced pluripotent stem cells (iPSCs) have an extensive application in regenerative medicine, pharmaceutical discovery, and basic research. With the recent derivation of rat iPSCs, it is now feasible to apply genetic manipulation in this… read more here.
Keywords: genetic manipulation; rat ipscs; pluripotent stem; induced pluripotent ... See more keywords