CRISPR/Cas9-based genetic screen of SCNT-reprogramming resistant genes identifies critical genes for male germ cell development in mice
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-94851-9
Abstract: Male germ cells undergo complex developmental processes eventually producing spermatozoa through spermatogenesis, although the molecular mechanisms remain largely elusive. We have previously identified somatic cell nuclear transfer-reprogramming resistant genes (SRRGs) that are highly enriched for… read more here.
Keywords: genetic screen; reprogramming resistant; mice; male germ ... See more keywords
A forward genetic screen in C. elegans identifies conserved residues of spliceosomal proteins PRP8 and SNRNP200/BRR2 with a role in maintaining 5′ splice site identity
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac991
Abstract: Abstract The spliceosome undergoes extensive rearrangements as it assembles onto precursor messenger RNAs. In the earliest assembly step, U1snRNA identifies the 5′ splice site. However, U1snRNA leaves the spliceosome relatively early in assembly, and 5′… read more here.
Keywords: snrnp200 brr2; genetic screen; forward genetic; site identity ... See more keywords
A functional genetic screen reveals sequence preferences within a key tertiary interaction in cobalamin riboswitches required for ligand selectivity
Sign Up to like & getrecommendations! Published in 2018 at "Nucleic Acids Research"
DOI: 10.1093/nar/gky539
Abstract: Abstract Riboswitches are structured mRNA sequences that regulate gene expression by directly binding intracellular metabolites. Generating the appropriate regulatory response requires the RNA rapidly and stably acquire higher-order structure to form the binding pocket, bind… read more here.
Keywords: functional genetic; cobalamin riboswitches; genetic screen; sequence ... See more keywords
A genetic screen for mutations affecting temperature sensing in Bacillus subtilis.
Sign Up to like & getrecommendations! Published in 2019 at "Microbiology"
DOI: 10.1099/mic.0.000741
Abstract: Two component systems, composed of a receptor histidine kinase and a cytoplasmic response regulator, regulate pivotal cellular processes in microorganisms. Here we describe a new screening procedure for the identification of amino acids that are… read more here.
Keywords: genetic screen; bacillus subtilis; screen mutations; temperature sensing ... See more keywords
Abstract 1360: A PARylation biosensor genetic screen to identify novel PARylation targets
Sign Up to like & getrecommendations! Published in 2018 at "Cancer Research"
DOI: 10.1158/1538-7445.am2018-1360
Abstract: Poly(ADP-ribosylation) (PARylation) is a reversible post-translational protein modification that regulates a number of cellular processes. It is generated by a family of PAR polymerases (PARP), of which PARP1 has recently been exploited as a target… read more here.
Keywords: novel parylation; identify novel; biosensor; parylation targets ... See more keywords
A genetic screen implicates a CWC16/Yju2/CCDC130 protein and SMU1 in alternative splicing in Arabidopsis thaliana.
Sign Up to like & getrecommendations! Published in 2017 at "RNA"
DOI: 10.1261/rna.060517.116
Abstract: To identify regulators of pre-mRNA splicing in plants, we developed a forward genetic screen based on an alternatively spliced GFP reporter gene in Arabidopsis thaliana In wild-type plants, three major splice variants issue from the… read more here.
Keywords: protein; cwc16 yju2; arabidopsis thaliana; gfp ... See more keywords
A forward genetic screen identifies Dolk as a regulator of startle magnitude through the potassium channel subunit Kv1.1
Sign Up to like & getrecommendations! Published in 2021 at "PLoS Genetics"
DOI: 10.1371/journal.pgen.1008943
Abstract: The acoustic startle response is an evolutionarily conserved avoidance behavior. Disruptions in startle behavior, particularly startle magnitude, are a hallmark of several human neurological disorders. While the neural circuitry underlying startle behavior has been studied… read more here.
Keywords: magnitude; startle behavior; channel subunit; forward genetic ... See more keywords
Unraveling Novel Mechanisms of Neurodegeneration Through a Large-Scale Forward Genetic Screen in Drosophila
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2018.00700
Abstract: Neurodegeneration is characterized by progressive loss of neurons. Genetic and environmental factors both contribute to demise of neurons, leading to diverse devastating cognitive and motor disorders, including Alzheimer’s and Parkinson’s diseases in humans. Over the… read more here.
Keywords: drosophila; large scale; neurodegeneration; genetic screen ... See more keywords