Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Cell"
DOI: 10.1016/j.molcel.2017.09.012
Abstract: Summary Chemical libraries paired with phenotypic screens can now readily identify compounds with therapeutic potential. A central limitation to exploiting these compounds, however, has been in identifying their relevant cellular targets. Here, we present a… read more here.
Keywords: based chemical; chemical genetic; combined crispri; genetic screens ... See more keywords
Genetic screens in isogenic mammalian cell lines without single cell cloning
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-14620-6
Abstract: Isogenic pairs of cell lines, which differ by a single genetic modification, are powerful tools for understanding gene function. Generating such pairs of mammalian cells, however, is labor-intensive, time-consuming, and, in some cell types, essentially… read more here.
Keywords: single cell; cell cloning; cell lines; genetic screens ... See more keywords
CRISPR/Cas9 and genetic screens in malaria parasites: small genomes, big impact
Sign Up to like & getrecommendations! Published in 2022 at "Biochemical Society Transactions"
DOI: 10.1042/bst20210281
Abstract: The ∼30 Mb genomes of the Plasmodium parasites that cause malaria each encode ∼5000 genes, but the functions of the majority remain unknown. This is due to a paucity of functional annotation from sequence homology, which… read more here.
Keywords: crispr cas9; screens malaria; malaria parasites; genetic screens ... See more keywords
Mutagenomics: The Future of Genetic Screens
Sign Up to like & getrecommendations! Published in 2020 at "Plant Physiology"
DOI: 10.1104/pp.20.01455
Abstract: Genetic screens are a remarkable way of identifying mutations affecting various aspects of plant growth and development. Arabidopsis ( Arabidopsis thaliana ) (T)-DNA insertion lines enable rapid identification of mutant genes but are tedious to… read more here.
Keywords: future genetic; physiology; mutagenomics future; genetic screens ... See more keywords
Quantitative genetic screening reveals a Ragulator-FLCN feedback loop that regulates the mTORC1 pathway
Sign Up to like & getrecommendations! Published in 2020 at "Science Signaling"
DOI: 10.1126/scisignal.aba5665
Abstract: A genetic screen in haploid human cells reveals context-specific regulators of the mTORC1 pathway. Perturb and observe screening in haploid cells Yeast have been a powerful model organism for genetic screens because they can easily… read more here.
Keywords: lamtor4; mtorc1 pathway; genetic screens; haploid ... See more keywords
PO-494 Using functional genetic screens to understand resistance to PARPi in BRCA-deficient tumours
Sign Up to like & getrecommendations! Published in 2018 at "ESMO Open"
DOI: 10.1136/esmoopen-2018-eacr25.511
Abstract: Introduction Error-free repair of double-strand DNA breaks is achieved by homologous recombination (HR), and both BRCA1 and BRCA2 are crucial for this process. Inactivating germline mutations of BRCA1 and BRCA2 genes predispose to breast and… read more here.
Keywords: functional genetic; deficient; parpi; resistance ... See more keywords
Abstract A26: Prediction of synergistic anticancer drug combinations by integrating chemical and genetic screens
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Cancer Therapeutics"
DOI: 10.1158/1538-8514.synthleth-a26
Abstract: Identification of effective drug combinations inhibiting multiple target genes that are essential for cancer cell survival is seen as an effective strategy to overcome drug resistance. Cellular pathways are often redundant, and network rewiring in… read more here.
Keywords: target; drug; genetic screens; integrating chemical ... See more keywords
A DNA-Free Editing Platform for Genetic Screens in Soybean via CRISPR/Cas9 Ribonucleoprotein Delivery
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.939997
Abstract: CRISPR/Cas9-based ribonucleoprotein (RNP)-mediated system has the property of minimizing the effects related to the unwanted introduction of vector DNA and random integration of recombinant DNA. Here, we describe a platform based on the direct delivery… read more here.
Keywords: platform; dna; cas9; genetic screens ... See more keywords
Forward Genetic Screens as Tools to Investigate Role and Mechanisms of EMT in Cancer
Sign Up to like & getrecommendations! Published in 2022 at "Cancers"
DOI: 10.3390/cancers14235928
Abstract: Simple Summary Forward genetic screens link genome modifications to phenotypes, and have been successfully employed to identify oncogenes, tumor suppressor genes and genes involved in metastasis or therapy resistance. In particular, transposon-based insertional mutagenesis screens… read more here.
Keywords: tools investigate; role; genetic screens; forward genetic ... See more keywords