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   Articles with "genetic screens" as a keyword



Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent

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recommendations! Published in 2017 at "Molecular Cell"

DOI: 10.1016/j.molcel.2017.09.012

Abstract: Summary Chemical libraries paired with phenotypic screens can now readily identify compounds with therapeutic potential. A central limitation to exploiting these compounds, however, has been in identifying their relevant cellular targets. Here, we present a… read more here.

Keywords: based chemical; chemical genetic; combined crispri; genetic screens ... See more keywords
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Genetic screens in isogenic mammalian cell lines without single cell cloning

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recommendations! Published in 2020 at "Nature Communications"

DOI: 10.1038/s41467-020-14620-6

Abstract: Isogenic pairs of cell lines, which differ by a single genetic modification, are powerful tools for understanding gene function. Generating such pairs of mammalian cells, however, is labor-intensive, time-consuming, and, in some cell types, essentially… read more here.

Keywords: single cell; cell cloning; cell lines; genetic screens ... See more keywords

CRISPR/Cas9 and genetic screens in malaria parasites: small genomes, big impact

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recommendations! Published in 2022 at "Biochemical Society Transactions"

DOI: 10.1042/bst20210281

Abstract: The ∼30 Mb genomes of the Plasmodium parasites that cause malaria each encode ∼5000 genes, but the functions of the majority remain unknown. This is due to a paucity of functional annotation from sequence homology, which… read more here.

Keywords: crispr cas9; screens malaria; malaria parasites; genetic screens ... See more keywords
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Mutagenomics: The Future of Genetic Screens

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recommendations! Published in 2020 at "Plant Physiology"

DOI: 10.1104/pp.20.01455

Abstract: Genetic screens are a remarkable way of identifying mutations affecting various aspects of plant growth and development. Arabidopsis ( Arabidopsis thaliana ) (T)-DNA insertion lines enable rapid identification of mutant genes but are tedious to… read more here.

Keywords: future genetic; physiology; mutagenomics future; genetic screens ... See more keywords

Quantitative genetic screening reveals a Ragulator-FLCN feedback loop that regulates the mTORC1 pathway

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recommendations! Published in 2020 at "Science Signaling"

DOI: 10.1126/scisignal.aba5665

Abstract: A genetic screen in haploid human cells reveals context-specific regulators of the mTORC1 pathway. Perturb and observe screening in haploid cells Yeast have been a powerful model organism for genetic screens because they can easily… read more here.

Keywords: lamtor4; mtorc1 pathway; genetic screens; haploid ... See more keywords

PO-494 Using functional genetic screens to understand resistance to PARPi in BRCA-deficient tumours

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recommendations! Published in 2018 at "ESMO Open"

DOI: 10.1136/esmoopen-2018-eacr25.511

Abstract: Introduction Error-free repair of double-strand DNA breaks is achieved by homologous recombination (HR), and both BRCA1 and BRCA2 are crucial for this process. Inactivating germline mutations of BRCA1 and BRCA2 genes predispose to breast and… read more here.

Keywords: functional genetic; deficient; parpi; resistance ... See more keywords

Abstract A26: Prediction of synergistic anticancer drug combinations by integrating chemical and genetic screens

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recommendations! Published in 2017 at "Molecular Cancer Therapeutics"

DOI: 10.1158/1538-8514.synthleth-a26

Abstract: Identification of effective drug combinations inhibiting multiple target genes that are essential for cancer cell survival is seen as an effective strategy to overcome drug resistance. Cellular pathways are often redundant, and network rewiring in… read more here.

Keywords: target; drug; genetic screens; integrating chemical ... See more keywords

Functional genetic screens reveal key pathways instructing the molecular phenotypes of tumor-associated macrophages.

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recommendations! Published in 2025 at "Cancer immunology research"

DOI: 10.1158/2326-6066.cir-25-0488

Abstract: Tumor-associated macrophages (TAMs) display remarkable functional heterogeneity, yet the molecular mechanisms driving their diverse phenotypes remain elusive. Using CRISPR screens in primary macrophages, we identified tumor-derived factors, including lactic acid, PGE2, and GM-CSF, as key… read more here.

Keywords: screens reveal; functional genetic; tumor associated; tumor ... See more keywords

Identifying Strategies to Improve Shared Decision Making for Pregnant Patients’ Decisions about Prenatal Genetic Screens and Diagnostic Tests

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recommendations! Published in 2024 at "Medical Decision Making"

DOI: 10.1177/0272989x241259016

Abstract: Purpose Prenatal genetic screens and diagnostic tests are vital components of prenatal care. The first prenatal visit is a critical time in the decision-making process when patients decide whether to use these tests in addition… read more here.

Keywords: diagnostic tests; decision making; prenatal genetic; decision ... See more keywords

A machine learning enhanced EMS mutagenesis probability map for efficient identification of causal mutations in Caenorhabditis elegans

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recommendations! Published in 2024 at "PLOS Genetics"

DOI: 10.1371/journal.pgen.1011377

Abstract: Chemical mutagenesis-driven forward genetic screens are pivotal in unveiling gene functions, yet identifying causal mutations behind phenotypes remains laborious, hindering their high-throughput application. Here, we reveal a non-uniform mutation rate caused by Ethyl Methane Sulfonate… read more here.

Keywords: mutagenesis probability; machine learning; learning enhanced; genetic screens ... See more keywords

A DNA-Free Editing Platform for Genetic Screens in Soybean via CRISPR/Cas9 Ribonucleoprotein Delivery

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recommendations! Published in 2022 at "Frontiers in Plant Science"

DOI: 10.3389/fpls.2022.939997

Abstract: CRISPR/Cas9-based ribonucleoprotein (RNP)-mediated system has the property of minimizing the effects related to the unwanted introduction of vector DNA and random integration of recombinant DNA. Here, we describe a platform based on the direct delivery… read more here.

Keywords: platform; dna; cas9; genetic screens ... See more keywords