Genetic tools to dissect functions of long noncoding RNAs
Sign Up to like & getrecommendations! Published in 2022 at "IUBMB Life"
DOI: 10.1002/iub.2690
Abstract: LncRNAs represent an abundant group of noncoding transcripts, some of which carry out important regulatory functions. To survey the biological and molecular roles of lncRNAs, reliable strategies for their genetic inactivation are required. Several lncRNA… read more here.
Keywords: long noncoding; functions long; tools dissect; dissect functions ... See more keywords
Molecular Biology and Genetic Tools to Investigate Functional Redundancy Among Fe-S Cluster Carriers in E. coli.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1605-5_1
Abstract: Iron-sulfur (Fe-S) clusters are among the oldest protein cofactors, and Fe-S cluster-based chemistry has shaped the cellular metabolism of all living organisms. Over the last 30 years, thanks to molecular biology and genetic approaches, numerous actors… read more here.
Keywords: cluster carriers; genetic tools; biology; molecular biology ... See more keywords
Novel genetic tools for probing individual H3 molecules in each nucleosome
Sign Up to like & getrecommendations! Published in 2018 at "Current Genetics"
DOI: 10.1007/s00294-018-0910-0
Abstract: In eukaryotes, genomic DNA is packaged into the nucleus together with histone proteins, forming chromatin. The fundamental repeating unit of chromatin is the nucleosome, a naturally symmetric structure that wraps DNA and is the substrate… read more here.
Keywords: individual molecules; novel genetic; genetic tools; molecules nucleosome ... See more keywords
Lactobacilli and pediococci as versatile cell factories - Evaluation of strain properties and genetic tools.
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2017.04.002
Abstract: This review discusses opportunities and bottlenecks for cell factory development of Lactic Acid Bacteria (LAB), with an emphasis on lactobacilli and pediococci, their metabolism and genetic tools. In order to enable economically feasible bio-based production… read more here.
Keywords: lactobacilli pediococci; production; cell factories; versatile cell ... See more keywords
Hacking nature: genetic tools for reprograming enzymes
Sign Up to like & getrecommendations! Published in 2017 at "Microbiology Australia"
DOI: 10.1071/ma17032
Abstract: Enzymes have many modern industrial applications, from biomass decomposition in the production of biofuels to highlystereospecificbiotransformations inpharmaceutical manufacture. The capacity to find or engineer enzymes with activities pertinent to specific applications has been essential for… read more here.
Keywords: reprograming enzymes; nature genetic; genetic tools; tools reprograming ... See more keywords
Metabolic engineering strategies to enable microbial electrosynthesis utilization of CO2: recent progress and challenges.
Sign Up to like & getrecommendations! Published in 2023 at "Critical reviews in biotechnology"
DOI: 10.1080/07388551.2023.2167065
Abstract: Microbial electrosynthesis (MES) is a promising technology that mainly utilizes microbial cells to convert CO2 into value-added chemicals using electrons provided by the cathode. However, the low electron transfer rate is a solid bottleneck hindering… read more here.
Keywords: metabolic engineering; engineering strategies; microbial electrosynthesis; electron transfer ... See more keywords
Genetic tools for the stable overexpression of circular RNAs
Sign Up to like & getrecommendations! Published in 2022 at "RNA Biology"
DOI: 10.1080/15476286.2022.2043041
Abstract: ABSTRACT Circular RNAs (circRNAs) are a class of non-coding RNAs featuring a covalently closed ring structure formed through backsplicing. circRNAs are broadly expressed and contribute to biological processes through a variety of functions. Standard gain-of-function… read more here.
Keywords: expression; circrna expression; overexpression; circular rnas ... See more keywords
Development of genetic tools for heterologous protein expression in a pentose‐utilizing environmental isolate of Pseudomonas putida
Sign Up to like & getrecommendations! Published in 2023 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.14205
Abstract: Pseudomonas putida has emerged as a promising host for the conversion of biomass‐derived sugars and aromatic intermediates into commercially relevant biofuels and bioproducts. Most of the strain development studies previously published have focused on P.… read more here.
Keywords: development; expression; biomass; genetic tools ... See more keywords
Toolbox for studying neurovascular coupling in vivo, with a focus on vascular activity and calcium dynamics in astrocytes
Sign Up to like & getrecommendations! Published in 2022 at "Neurophotonics"
DOI: 10.1117/1.nph.9.2.021909
Abstract: Abstract. Significance: Insights into the cellular activity of each member of the neurovascular unit (NVU) is critical for understanding their contributions to neurovascular coupling (NVC)—one of the key control mechanisms in cerebral blood flow regulation.… read more here.
Keywords: neurovascular coupling; genetic tools; activity; vascular activity ... See more keywords
A Genetic Study of Nif-Associated Genes in a Hyperthermophilic Methanogen
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DOI: 10.1128/spectrum.02093-21
Abstract: Methanocaldococcus has been considered a model genus, but genetic tools have not been forthcoming until recently. Here, we develop and illustrate the utility of positive selection with either of two selective agents (simvastatin and neomycin),… read more here.
Keywords: deletion; markerless frame; hyperthermophilic methanogen; genetic tools ... See more keywords
Tracking Adventitial Fibroblast Contribution to Disease: A Review of Current Methods to Identify Resident Fibroblasts.
Sign Up to like & getrecommendations! Published in 2017 at "Arteriosclerosis, thrombosis, and vascular biology"
DOI: 10.1161/atvbaha.117.308199
Abstract: Cells present in the adventitia, or outermost layer of the blood vessel, contribute to the progression of vascular diseases, such as atherosclerosis, hypertension, and aortic dissection. The adventitial fibroblast of the aorta is the prototypic… read more here.
Keywords: fibroblast contribution; genetic tools; adventitial fibroblast; tracking adventitial ... See more keywords