An Oxidative Bioconjugation Strategy Targeted to a Genetically Encoded 5‐Hydroxytryptophan
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201800111
Abstract: Approaches that enable the chemoselective, covalent modification of proteins in a site‐specific manner have emerged as a powerful technology for a wide range of applications. The electron‐rich unnatural amino acid 5‐hydroxytryptophan was recently genetically encoded… read more here.
Keywords: bioconjugation strategy; oxidative bioconjugation; genetically encoded; strategy targeted ... See more keywords
Super‐Resolution Microscopy and Single‐Protein Tracking in Live Bacteria Using a Genetically Encoded, Photostable Fluoromodule
Sign Up to like & getrecommendations! Published in 2017 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.21
Abstract: Visualization of dynamic protein structures in live cells is crucial for understanding the mechanisms governing biological processes. Fluorescence microscopy is a sensitive tool for this purpose. In order to image proteins in live bacteria using… read more here.
Keywords: protein; encoded photostable; microscopy; genetically encoded ... See more keywords
Applications of genetically encoded photosensitizer miniSOG: from correlative light electron microscopy to immunophotosensitizing.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of biophotonics"
DOI: 10.1002/jbio.201600120
Abstract: Genetically encoded photosensitizers (PSs), e.g. ROS generating proteins, correspond to a novel class of PSs that are highly desirable for biological and medical applications since they can be used in combination with a variety of… read more here.
Keywords: microscopy; genetically encoded; correlative light; electron microscopy ... See more keywords
Structural diversity in de novo cyclic peptide ligands from genetically encoded library technologies
Sign Up to like & getrecommendations! Published in 2020 at "Peptide Science"
DOI: 10.1002/pep2.24204
Abstract: Cyclic peptides discovered by genetically encoded library technologies have emerged as a class of promising molecules in chemical biology and drug discovery. Here we review the cyclic peptides identified through these techniques reported in the… read more here.
Keywords: library technologies; cyclic peptide; encoded library; genetically encoded ... See more keywords
Imaging of Genetically Encoded FRET-Based Biosensors to Detect GPCR Activity.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1221-7_11
Abstract: A wealth of assays for screening GPCR activity have been developed. Biosensors that employ Förster Resonance Energy transfer (FRET) are specific and enable dynamic measurements. Moreover, FRET biosensors are ideally suited for the analysis of… read more here.
Keywords: gpcr activity; fret based; genetically encoded; biosensors detect ... See more keywords
Genetically Encoded Biosensors for the Quantitative Analysis of Auxin Dynamics in Plant Cells.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1791-5_11
Abstract: Plants, as sessile organisms, possess complex and intertwined signaling networks to react and adapt their behavior toward different internal and external stimuli. Due to this high level of complexity, the implementation of quantitative molecular tools… read more here.
Keywords: genetically encoded; analysis auxin; auxin; biology ... See more keywords
Probing Protein-Protein Interactions with Genetically Encoded Photoactivatable Cross-Linkers.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7240-1_26
Abstract: Fundamental to all living organisms is the ability of proteins to interact with other biological molecules at the right time and location, with the proper affinity, and to do so reversibly. One well-established technique to… read more here.
Keywords: protein; protein interactions; photoactivatable cross; genetically encoded ... See more keywords
Visualization of Intracellular Hydrogen Peroxide with the Genetically Encoded Fluorescent Probe HyPer in NIH-3T3 Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9424-3_15
Abstract: Reactive oxygen species (ROS) are involved in regulating normal physiological cell functions as second messengers as well as nonspecific damage of biomolecules in a pathological process known as oxidative stress. The HyPer family of genetically… read more here.
Keywords: nih 3t3; probe; hydrogen peroxide; genetically encoded ... See more keywords
Visualisation of an nsPEF induced calcium wave using the genetically encoded calcium indicator GCaMP in U87 human glioblastoma cells.
Sign Up to like & getrecommendations! Published in 2018 at "Bioelectrochemistry"
DOI: 10.1016/j.bioelechem.2017.09.003
Abstract: Cytosolic, synthetic chemical calcium indicators are typically used to visualise the rapid increase in intracellular calcium ion concentration that follows nanosecond pulsed electric field (nsPEF) application. This study looks at the application of genetically encoded… read more here.
Keywords: encoded calcium; nspef induced; wave; genetically encoded ... See more keywords
Development of a convenient and supersensitive high-throughput screening system for genetically encoded fluorescent probes of small molecules using a confocal microscope.
Sign Up to like & getrecommendations! Published in 2017 at "Cell calcium"
DOI: 10.1016/j.ceca.2016.09.007
Abstract: Monitoring the dynamic patterns of intracellular signaling molecules, such as inositol 1,4,5-trisphosphate (IP3) and Ca2+, that control many diverse cellular processes, provides us significant information to understand the regulatory mechanism of cellular functions. For searching… read more here.
Keywords: detection; system; genetically encoded; convenient supersensitive ... See more keywords
Comparative Evaluation of Genetically Encoded Voltage Indicators
Sign Up to like & getrecommendations! Published in 2019 at "Cell reports"
DOI: 10.1016/j.celrep.2018.12.088
Abstract: SUMMARY Imaging voltage using fluorescent-based sensors could be an ideal technique to probe neural circuits with high spatiotemporal resolution. However, due to insufficient signal-to-noise ratio (SNR), imaging membrane potential in mammalian preparations is still challenging.… read more here.
Keywords: voltage; voltage indicators; photon; encoded voltage ... See more keywords