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Published in 2022 at "Microbiology and Immunology"
DOI: 10.1111/1348-0421.13032
Abstract: The reverse genetics system is a very powerful tool for analyzing the molecular mechanisms of viral propagation and pathogenesis. However, full‐length genome plasmid construction is highly time‐consuming and laborious, and undesired mutations may be introduced…
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Keywords:
mumps virus;
using mumps;
genetics system;
reverse genetics ... See more keywords
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Published in 2019 at "Journal of Virology"
DOI: 10.1128/jvi.00963-19
Abstract: Reverse genetics, an approach used to generate viruses from cloned cDNA, has increased our understanding of virus biology. Worldwide research led to the development of an entirely plasmid-based reverse genetics system for the simian RV…
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Keywords:
reverse genetics;
genetics system;
genetics;
biology ... See more keywords
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Published in 2022 at "mBio"
DOI: 10.1128/mbio.01308-22
Abstract: Rotaviruses represent one of the most successful pathogens in the world, with high infectivity and efficient transmission between the young of many animal species, including humans. To overcome host defenses, rotaviruses have evolved a plethora…
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Keywords:
immune evasion;
innate immune;
genetics system;
reverse genetics ... See more keywords
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Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2023.1141101
Abstract: Introduction Reverse genetics has become an indispensable tool to gain insight into the pathogenesis of viruses and the development of vaccines. The yeast-based synthetic genomics platform has demonstrated the novel capabilities to genetically reconstruct different…
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Keywords:
system;
development;
genetics system;
reverse genetics ... See more keywords
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Published in 2020 at "Viruses"
DOI: 10.3390/v12030301
Abstract: A novel fowl adenovirus 4 (FAdV-4) has caused significant economic losses to the poultry industry in China since 2015. We established an easy-to-use reverse genetics system for modification of the whole right and partial left…
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Keywords:
system modification;
fadv4;
reverse genetics;
genetics ... See more keywords
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Published in 2020 at "Viruses"
DOI: 10.3390/v12040400
Abstract: The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to…
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Keywords:
reverse genetics;
genetics;
toscana virus;
genetics system ... See more keywords
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Published in 2020 at "Viruses"
DOI: 10.3390/v12070767
Abstract: Canine adenoviruses (CAdVs) are divided into pathotypes CAdV1 and CAdV2, which cause infectious hepatitis and laryngotracheitis in canid animals, respectively. They can be the backbones of viral vectors that could be applied in recombinant vaccines…
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Keywords:
reverse genetics;
genetics;
genetics system;
canine adenoviruses ... See more keywords