Demonstration of bacterium‐free very rapid reverse genetics system using mumps virus
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology and Immunology"
DOI: 10.1111/1348-0421.13032
Abstract: The reverse genetics system is a very powerful tool for analyzing the molecular mechanisms of viral propagation and pathogenesis. However, full‐length genome plasmid construction is highly time‐consuming and laborious, and undesired mutations may be introduced… read more here.
Keywords: mumps virus; using mumps; genetics system; reverse genetics ... See more keywords
Reverse Genetics System for a Human Group A Rotavirus
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Virology"
DOI: 10.1128/jvi.00963-19
Abstract: Reverse genetics, an approach used to generate viruses from cloned cDNA, has increased our understanding of virus biology. Worldwide research led to the development of an entirely plasmid-based reverse genetics system for the simian RV… read more here.
Keywords: reverse genetics; genetics system; genetics; biology ... See more keywords
Rapid production of recombinant rotaviruses by overexpression of NSP2 and NSP5 genes with modified nucleotide sequences
Sign Up to like & getrecommendations! Published in 2024 at "Journal of Virology"
DOI: 10.1128/jvi.00996-24
Abstract: ABSTRACT Reverse genetics systems for rotaviruses (RV) facilitate the generation of genetically engineered RVs by transfection of 11 plasmids encoding 11 genomic viral RNA segments. In addition to viral genome expression, overexpression of NSP2 and… read more here.
Keywords: genetics; genetics system; reverse genetics; nsp2 nsp5 ... See more keywords
Re-Examining Rotavirus Innate Immune Evasion: Potential Applications of the Reverse Genetics System
Sign Up to like & getrecommendations! Published in 2022 at "mBio"
DOI: 10.1128/mbio.01308-22
Abstract: Rotaviruses represent one of the most successful pathogens in the world, with high infectivity and efficient transmission between the young of many animal species, including humans. To overcome host defenses, rotaviruses have evolved a plethora… read more here.
Keywords: immune evasion; innate immune; genetics system; reverse genetics ... See more keywords
Generation of an artificially attenuated fowl adenovirus 4 viral vector using the reverse genetics system based on full-length infectious clone
Sign Up to like & getrecommendations! Published in 2025 at "Veterinary Research"
DOI: 10.1186/s13567-025-01496-x
Abstract: Fowl adenovirus serotype 4 (FAdV-4) is a non-enveloped double-stranded DNA virus with a 43–45 kb genome. This characteristic makes it a promising viral vector for expressing other antigens in developing multi-valent or multi-series vaccines in… read more here.
Keywords: genetics; reverse genetics; infectious clone; genetics system ... See more keywords
Development of a rapid reverse genetics system for feline coronavirus based on TAR cloning in yeast
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2023.1141101
Abstract: Introduction Reverse genetics has become an indispensable tool to gain insight into the pathogenesis of viruses and the development of vaccines. The yeast-based synthetic genomics platform has demonstrated the novel capabilities to genetically reconstruct different… read more here.
Keywords: system; development; genetics system; reverse genetics ... See more keywords
User-Friendly Reverse Genetics System for Modification of the Right End of Fowl Adenovirus 4 Genome
Sign Up to like & getrecommendations! Published in 2020 at "Viruses"
DOI: 10.3390/v12030301
Abstract: A novel fowl adenovirus 4 (FAdV-4) has caused significant economic losses to the poultry industry in China since 2015. We established an easy-to-use reverse genetics system for modification of the whole right and partial left… read more here.
Keywords: system modification; fadv4; reverse genetics; genetics ... See more keywords
Novel Toscana Virus Reverse Genetics System Establishes NSs as an Antagonist of Type I Interferon Responses
Sign Up to like & getrecommendations! Published in 2020 at "Viruses"
DOI: 10.3390/v12040400
Abstract: The sand fly-borne Toscana virus (TOSV) is the major cause of human meningoencephalitis in the Mediterranean basin during the summer season. In this work, we have developed a T7 RNA polymerase-driven reverse genetics system to… read more here.
Keywords: reverse genetics; genetics; toscana virus; genetics system ... See more keywords
Establishment of a Simple and Efficient Reverse Genetics System for Canine Adenoviruses Using Bacterial Artificial Chromosomes
Sign Up to like & getrecommendations! Published in 2020 at "Viruses"
DOI: 10.3390/v12070767
Abstract: Canine adenoviruses (CAdVs) are divided into pathotypes CAdV1 and CAdV2, which cause infectious hepatitis and laryngotracheitis in canid animals, respectively. They can be the backbones of viral vectors that could be applied in recombinant vaccines… read more here.
Keywords: reverse genetics; genetics; genetics system; canine adenoviruses ... See more keywords