Deep targeted sequencing of cytological tumor cells using whole genome amplification
Sign Up to like & getrecommendations! Published in 2022 at "Cancer Cytopathology"
DOI: 10.1002/cncy.22653
Abstract: Genomic profiling in lung cancer is essential for precision medicine. Cytological specimens provide an alternative to formalin‐fixed paraffin‐embedded (FFPE) samples for comprehensive genomic analysis. However, this approach remains challenging when a limited number of tumor… read more here.
Keywords: whole genome; genome amplification; tumor cells; deep targeted ... See more keywords
Cost‐saving population genomic investigation of Daphnia longispina complex resting eggs using whole‐genome amplification and pre‐sequencing screening
Sign Up to like & getrecommendations! Published in 2022 at "Ecology and Evolution"
DOI: 10.1002/ece3.9682
Abstract: Abstract Resting stages of aquatic organisms that accumulate in the sediment over time are an exceptional resource that allows direct insights into past populations and addressing evolutionary questions. This is of particular interest in taxa… read more here.
Keywords: genome amplification; daphnia longispina; resting eggs; daphnia ... See more keywords
Testing the impact of Whole Genome Amplification on genome comparison using the polyploid flagellated Giardia duodenalis as a model.
Sign Up to like & getrecommendations! Published in 2019 at "Experimental parasitology"
DOI: 10.1016/j.exppara.2019.107776
Abstract: The availability of high quality genomic DNA in sufficient amounts to perform Next Generation Sequencing (NGS) experiments is challenging for pathogens that cannot be cultivated in vitro, as is the case for many parasites. Therefore,… read more here.
Keywords: whole genome; genomic dna; duodenalis model; genome amplification ... See more keywords
A comparative study of whole genome amplification and low-template DNA profiling
Sign Up to like & getrecommendations! Published in 2019 at "Forensic Science International: Genetics Supplement Series"
DOI: 10.1016/j.fsigss.2019.10.070
Abstract: Abstract Generation of high-quality STR profiles from trace amounts of cells remains challenging. Although increasing the number of PCR cycles improves the sensitivity of STR analysis, stochastic effects may complicate interpretation and deem the profiles… read more here.
Keywords: whole genome; dna; str; cycle ... See more keywords
1D-Reactor Decentralized MDA for Uniform and Accurate Whole Genome Amplification.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b02183
Abstract: Multiple displacement amplification (MDA), a most popular isothermal whole genome amplification (WGA) method, suffers the major hurdle of highly uneven amplification, thus, leading to many problems in approaching biological applications related to copy-number assessment. In… read more here.
Keywords: whole genome; mda uniform; reactor decentralized; genome amplification ... See more keywords
Characterizing Complex Populations of Endogenous Adeno-associated Viruses by Single-Genome Amplification.
Sign Up to like & getrecommendations! Published in 2022 at "Human gene therapy"
DOI: 10.1089/hum.2022.099
Abstract: The isolation of adeno-associated virus (AAV) genomes from biomaterials at the molecular level has traditionally relied on polymerase chain reaction-based and cloning-based techniques. However, when applied to samples containing multiple species, traditional techniques for isolating… read more here.
Keywords: genome amplification; single genome; aav; aav sga ... See more keywords
Whole genome amplification and sequencing of a Daphnia resting egg
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Ecology Resources"
DOI: 10.1111/1755-0998.12720
Abstract: Resting eggs banks are unique windows that allow us to directly observe shifts in population genetics, and phenotypes over time as natural populations evolve. Though a variety of planktonic organisms also produce resting stages, the… read more here.
Keywords: dna; daphnia; egg; genome amplification ... See more keywords
Impact of Contaminating DNA in Whole-Genome Amplification Kits Used for Metagenomic Shotgun Sequencing for Infection Diagnosis
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Clinical Microbiology"
DOI: 10.1128/jcm.02402-16
Abstract: ABSTRACT Whole-genome amplification (WGA) is a useful tool for amplification of very small quantities of DNA for many uses, including metagenomic shotgun sequencing for infection diagnosis. Depending on the application, background DNA from WGA kits… read more here.
Keywords: whole genome; sequencing infection; shotgun sequencing; metagenomic shotgun ... See more keywords
Microfluidics-Based Enrichment and Whole-Genome Amplification Enable Strain-Level Resolution for Airway Metagenomics
Sign Up to like & getrecommendations! Published in 2019 at "mSystems"
DOI: 10.1128/msystems.00198-19
Abstract: The airway microbial community, which takes important pathogenic roles for respiratory diseases, is far from clear in terms of taxonomy and gene functions. One of the critical reasons is the heavy contamination of host cell/DNA… read more here.
Keywords: whole genome; based enrichment; genome amplification; microfluidics based ... See more keywords
Abstract 5401: Assessment of whole genome amplification for whole exome sequencing in detecting genetic mutation
Sign Up to like & getrecommendations! Published in 2017 at "Cancer Research"
DOI: 10.1158/1538-7445.am2017-5401
Abstract: Whole exome sequencing (WES) has been widely used for studying genetic mutations in DNA coding regions to elucidate cancer etiology and for identifying biomarkers to optimize chemotherapy. However, these studies can be limited when only… read more here.
Keywords: exome sequencing; whole genome; genome amplification; whole exome ... See more keywords
Whole-genome amplification/preimplantation genetic testing for propionic acidemia of successful pregnancy in an obligate carrier Mexican couple: A case report
Sign Up to like & getrecommendations! Published in 2021 at "World Journal of Clinical Cases"
DOI: 10.12998/wjcc.v9.i29.8797
Abstract: BACKGROUND Identifying a potential single monogenetic disorder in healthy couples is costly due to the Assisted Reproduction facilities' current methodology for screening, which focuses on the detecting multiple genetic disorders at once. Here, we report… read more here.
Keywords: couple; genetic testing; whole genome; preimplantation genetic ... See more keywords