The Delivery Challenge of Genome Editing in Human Epithelia.
Sign Up to like & getrecommendations! Published in 2021 at "Advanced healthcare materials"
DOI: 10.1002/adhm.202100847
Abstract: Despite exciting advances in gene editing, their clinical translation is still hampered by the lack of delivery systems that can encapsulate and deliver gene editing tools like CRISPR-Cas9 or prime editors to the target side.… read more here.
Keywords: genome editing; gene editing; human epithelia; delivery ... See more keywords
Effective Genome Editing Using CRISPR‐Cas9 Nanoflowers
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DOI: 10.1002/adhm.202102365
Abstract: CRISPR‐Cas9 as a powerful gene‐editing tool has tremendous potential for the treatment of genetic diseases. Herein, a new mesoporous nanoflower (NF)‐like delivery nanoplatform termed Cas9‐NF is reported by crosslinking Cas9 and polymeric micelles that enables… read more here.
Keywords: genome editing; crispr cas9; effective genome; editing using ... See more keywords
A Multiple Targeting Genome Editing System for Remodulation of Circulating Malignant Cells to Eliminate Cancer Immunosuppression and Restore Immune Responses
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DOI: 10.1002/adhm.202401223
Abstract: Cancer immunotherapy, which aims to eliminate cancer immunosuppression and reactivate anticancer immunity, holds great promise in oncology treatments. However, it is challenging to accurately study the efficacy of immunotherapy based on human‐derived cells through animal… read more here.
Keywords: cancer; cancer immunosuppression; circulating malignant; eliminate cancer ... See more keywords
Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles.
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DOI: 10.1002/adma.201902575
Abstract: A main challenge to broaden the biomedical application of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9) genome editing technique is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the… read more here.
Keywords: genome editing; crispr; cas9 genome; crispr cas9 ... See more keywords
Rationally Engineering Pro‐Proteins and Membrane‐Penetrating α‑Helical Polypeptides for Genome Editing Toward Choroidal Neovascularization Treatment
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DOI: 10.1002/adma.202412366
Abstract: Ribonucleoprotein (RNP)‐based CRISPR/Cas9 genome editing holds great potential for the treatment of choroidal neovascularization (CNV), which however, is challenged by the lack of efficient cytosolic protein delivery tools. Herein, reversibly‐phosphorylated pro‐proteins (P‐proteins) with conjugated adenosine… read more here.
Keywords: treatment; membrane penetrating; genome editing; pro proteins ... See more keywords
CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects
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DOI: 10.1002/advs.201902312
Abstract: Abstract Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas (CRISPR‐associated) systems, which make the targeted modification of… read more here.
Keywords: genome editing; target effects; crispr cas; target ... See more keywords
Magnetic Nanoparticle‐Assisted Non‐Viral CRISPR‐Cas9 for Enhanced Genome Editing to Treat Rett Syndrome
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DOI: 10.1002/advs.202306432
Abstract: The CRISPR‐Cas9 technology has the potential to revolutionize the treatment of various diseases, including Rett syndrome, by enabling the correction of genes or mutations in human patient cells. However, several challenges need to be addressed… read more here.
Keywords: crispr; rett syndrome; genome editing; magnetic nanoparticle ... See more keywords
Engineering of Zinc Finger Nucleases Through Structural Modeling Improves Genome Editing Efficiency in Cells
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DOI: 10.1002/advs.202310255
Abstract: Genome Editing is widely used in biomedical research and medicine. Zinc finger nucleases (ZFNs) are smaller in size than transcription activator‐like effector (TALE) nucleases (TALENs) and CRISPR‐Cas9. Therefore, ZFN‐encoding DNAs can be easily packaged into… read more here.
Keywords: genome editing; zinc finger; zfns; editing efficiency ... See more keywords
From Code to Life: The AI‐Driven Revolution in Genome Editing
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DOI: 10.1002/advs.202417029
Abstract: Genome editing has revolutionized modern biotechnology, enabling precise modifications to DNA sequences with far‐reaching applications in medicine, agriculture, and synthetic biology. Recent advancements in artificial intelligence (AI) have significantly enhanced genome editing by improving target… read more here.
Keywords: code life; driven revolution; genome; medicine ... See more keywords
Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects
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DOI: 10.1002/bies.202200032
Abstract: Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have… read more here.
Keywords: genome editing; crispr; prime editing; plants mammalian ... See more keywords
Breakthrough in CRISPR/Cas system: Current and future directions and challenges.
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DOI: 10.1002/biot.202200642
Abstract: Targeted genome editing (GE) technology has brought a significant revolution in fictional genomic research and given hope to plant scientists to develop desirable varieties. This technology involves inducing site-specific DNA perturbations that can be repaired… read more here.
Keywords: genome editing; technology; crispr cas; crispr ... See more keywords