The Delivery Challenge of Genome Editing in Human Epithelia.
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DOI: 10.1002/adhm.202100847
Abstract: Despite exciting advances in gene editing, their clinical translation is still hampered by the lack of delivery systems that can encapsulate and deliver gene editing tools like CRISPR-Cas9 or prime editors to the target side.… read more here.
Keywords: genome editing; gene editing; human epithelia; delivery ... See more keywords
Effective Genome Editing Using CRISPR‐Cas9 Nanoflowers
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DOI: 10.1002/adhm.202102365
Abstract: CRISPR‐Cas9 as a powerful gene‐editing tool has tremendous potential for the treatment of genetic diseases. Herein, a new mesoporous nanoflower (NF)‐like delivery nanoplatform termed Cas9‐NF is reported by crosslinking Cas9 and polymeric micelles that enables… read more here.
Keywords: genome editing; crispr cas9; effective genome; editing using ... See more keywords
Fast and Efficient CRISPR/Cas9 Genome Editing In Vivo Enabled by Bioreducible Lipid and Messenger RNA Nanoparticles.
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DOI: 10.1002/adma.201902575
Abstract: A main challenge to broaden the biomedical application of CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat (CRISPR) associated protein 9) genome editing technique is the delivery of Cas9 nuclease and single-guide RNA (sgRNA) into the… read more here.
Keywords: genome editing; crispr; cas9 genome; crispr cas9 ... See more keywords
CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects
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DOI: 10.1002/advs.201902312
Abstract: Abstract Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas (CRISPR‐associated) systems, which make the targeted modification of… read more here.
Keywords: genome editing; target effects; crispr cas; target ... See more keywords
Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects
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DOI: 10.1002/bies.202200032
Abstract: Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have… read more here.
Keywords: genome editing; crispr; prime editing; plants mammalian ... See more keywords
Breakthrough in CRISPR/Cas system: Current and future directions and challenges.
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DOI: 10.1002/biot.202200642
Abstract: Targeted genome editing (GE) technology has brought a significant revolution in fictional genomic research and given hope to plant scientists to develop desirable varieties. This technology involves inducing site-specific DNA perturbations that can be repaired… read more here.
Keywords: genome editing; technology; crispr cas; crispr ... See more keywords
Exploiting heterologous and endogenous CRISPR‐Cas systems for genome editing in the probiotic Clostridium butyricum
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DOI: 10.1002/bit.27753
Abstract: Clostridium butyricum has been widely used as a probiotic for humans and food animals. However, the mechanisms of beneficial effects of C. butyricum on the host remain poorly understood, largely due to the lack of… read more here.
Keywords: genome editing; butyricum; crispr; crispr cas ... See more keywords
Genome editing technologies, mechanisms and improved production of therapeutic phytochemicals: Opportunities and prospects
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DOI: 10.1002/bit.28260
Abstract: Plants produce a large number of secondary metabolites, known as phytometabolites that may be employed as medicines, dyes, poisons, and insecticides in the field of medicine, agriculture, and industrial use, respectively. The rise of genome… read more here.
Keywords: genome editing; production therapeutic; technologies mechanisms; editing technologies ... See more keywords
Exploring the potential of CRISPR/Cas genome editing for vegetable crop improvement: An overview of challenges and approaches
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DOI: 10.1002/bit.28344
Abstract: Vegetables provide many nutrients in the form of fiber, vitamins, and minerals, which make them an important part of our diet. Numerous biotic and abiotic stresses can affect crop growth, quality, and yield. Traditional and… read more here.
Keywords: genome editing; crop improvement; crispr;
Expanding the CRISPR toolbox for Chinese hamster ovary cells with comprehensive tools for Mad7 genome editing
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28367
Abstract: The production of high‐value biopharmaceuticals is dominated by mammalian production cells, particularly Chinese hamster ovary (CHO) cells, which have been widely used and preferred in manufacturing processes. The discovery of CRISPR‐Cas9 significantly accelerated cell line… read more here.
Keywords: genome editing; crispr toolbox; crispr; chinese hamster ... See more keywords
Genome Editing in Mice Using CRISPR/Cas9 Technology
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DOI: 10.1002/cpcb.57
Abstract: CRISPR/Cas9 technology has revolutionized genome editing in mice, allowing for simple and rapid development of knockouts and knockins. CRISPR relies on small guide RNAs that direct the RNA‐guided nuclease Cas9 to a designated genomic site… read more here.
Keywords: genome editing; crispr cas9; cas9 technology; cas9 ... See more keywords