CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0290-4_24
Abstract: The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells… read more here.
Keywords: primary human; crispr cas9; engineering primary; genome engineering ... See more keywords
Conjugative Assembly Genome Engineering (CAGE).
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DOI: 10.1007/978-1-4939-9877-7_28
Abstract: Conjugative assembly genome engineering (CAGE) enables the transfer of large chromosomal regions from a donor to a recipient. Specific regions of the donor chromosome can be introduced in the recipient genome by the directed insertion… read more here.
Keywords: conjugative assembly; assembly genome; engineering cage; cage ... See more keywords
From Reductionism to Holism: Toward a More Complete View of Development Through Genome Engineering.
Sign Up to like & getrecommendations! Published in 2017 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-319-63904-8_3
Abstract: Paradigm shifts in science are often coupled to technological advances. New techniques offer new roads of discovery; but, more than this, they shape the way scientists approach questions. Developmental biology exemplifies this idea both in… read more here.
Keywords: reductionism; biology; holism; complete view ... See more keywords
In Vivo Genome Engineering for the Treatment of Muscular Dystrophies
Sign Up to like & getrecommendations! Published in 2020 at "Current Stem Cell Reports"
DOI: 10.1007/s40778-020-00173-3
Abstract: Purpose of Review Muscular dystrophies (MDs) are a heterogeneous collection of inherited disorders which cause progressive muscle loss and weakness/hypotonia. Owing to the genetic root of MDs, CRISPR/Cas9 genome editing has been investigated as a… read more here.
Keywords: crispr; crispr cas9; vivo genome; muscular dystrophies ... See more keywords
Application of CRISPR-mediated genome engineering in cancer research.
Sign Up to like & getrecommendations! Published in 2017 at "Cancer letters"
DOI: 10.1016/j.canlet.2016.03.029
Abstract: Cancer is a multistep process that arises from a series of genetic and epigenetic events. With recent technological advances there has been a burst in genome sequencing and epigenetic studies revealing a plethora of alterations… read more here.
Keywords: cancer; application crispr; cancer research; genome engineering ... See more keywords
Modern methods for laboratory diversification of biomolecules.
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2017.10.010
Abstract: Genetic variation fuels Darwinian evolution, yet spontaneous mutation rates are maintained at low levels to ensure cellular viability. Low mutation rates preclude the exhaustive exploration of sequence space for protein evolution and genome engineering applications,… read more here.
Keywords: laboratory diversification; evolution; diversification; modern methods ... See more keywords
Genome engineering in human pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2017 at "Current opinion in chemical engineering"
DOI: 10.1016/j.coche.2016.12.002
Abstract: Human pluripotent stem cells (hPSCs) hold great promise for modeling and recapitulation of human biological processes. Coupled with the advent of genome engineering tools, specifically the CRISPR–Cas9 systems, hPSCs have opened a multitude of possibilities… read more here.
Keywords: engineering; human pluripotent; pluripotent stem; stem cells ... See more keywords
Multiplexed site-specific genome engineering for overproducing bioactive secondary metabolites in actinomycetes.
Sign Up to like & getrecommendations! Published in 2017 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2017.01.004
Abstract: Actinomycetes produce a large variety of pharmaceutically active compounds, yet production titers often require to be improved for discovery, development and large-scale manufacturing. Here, we describe a new technique, multiplexed site-specific genome engineering (MSGE) via… read more here.
Keywords: multiplexed site; engineering; specific genome; site specific ... See more keywords
Genome Engineering for Next-Generation Cellular Immunotherapies.
Sign Up to like & getrecommendations! Published in 2022 at "Biochemistry"
DOI: 10.1021/acs.biochem.2c00340
Abstract: Over the past decade, cellular immunotherapies such as CAR-T, TCR-T, and NK cell therapies have achieved tremendous success in cancer treatment. However, various challenges and obstacles remain, including antigen escape, immunosuppression in the tumor microenvironment,… read more here.
Keywords: genome engineering; cellular immunotherapies; engineering next; next generation ... See more keywords
Diverse Class 2 CRISPR-Cas Effector Proteins for Genome Engineering Applications.
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DOI: 10.1021/acschembio.7b00800
Abstract: CRISPR-Cas genome editing technologies have revolutionized modern molecular biology by making targeted DNA edits simple and scalable. These technologies are developed by domesticating naturally occurring microbial adaptive immune systems that display wide diversity of functionality… read more here.
Keywords: crispr cas; effector; biology; genome engineering ... See more keywords
A RAGE Based Strategy for the Genome Engineering of the Human Respiratory Pathogen Mycoplasma pneumoniae.
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DOI: 10.1021/acssynbio.0c00263
Abstract: Genome engineering of microorganisms has become a standard in microbial biotechnologies. Several efficient tools are available for the genetic manipulation of model bacteria such as Escherichia coli and Bacillus subtilis, or the yeast Saccharomyces cerevisiae.… read more here.
Keywords: biology; mycoplasma pneumoniae; strategy; genome ... See more keywords