Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00366
Abstract: There is a growing interest in establishing the methylotrophic yeast Pichia pastoris as microbial cell factories for producing fuels, chemicals, and natural products, particularly with methanol as the feedstock. Although CRISPR (Clustered Regularly Interspaced Short… read more here.
Keywords: recombination; integration; homology arms; efficiency ... See more keywords
Proteome and genome integration analysis of obesity
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DOI: 10.1097/cm9.0000000000002644
Abstract: Abstract The prevalence of obesity has increased worldwide in recent decades. Genetic factors are now known to play a substantial role in the predisposition to obesity and may contribute up to 70% of the risk… read more here.
Keywords: proteome genome; integration analysis; analysis; obesity ... See more keywords
A plasmid toolset for CRISPR‐mediated genome editing and CRISPRi gene regulation in Escherichia coli
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DOI: 10.1111/1751-7915.13780
Abstract: CRISPR technologies have become standard laboratory tools for genetic manipulations across all kingdoms of life. Despite their origins in bacteria, the development of CRISPR tools for engineering bacteria has been slower than for eukaryotes; nevertheless,… read more here.
Keywords: gene regulation; genome editing; genome integration; crispr ... See more keywords
High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration
Sign Up to like & getrecommendations! Published in 2023 at "Science Advances"
DOI: 10.1126/sciadv.ade1285
Abstract: Efficient genome engineering is critical to understand and use microbial functions. Despite recent development of tools such as CRISPR-Cas gene editing, efficient integration of exogenous DNA with well-characterized functions remains limited to model bacteria. Here,… read more here.
Keywords: integration; specific genome; high throughput; site specific ... See more keywords