Articles with "genome integration" as a keyword



Enhancing Homologous Recombination Efficiency in Pichia pastoris for Multiplex Genome Integration Using Short Homology Arms.

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Published in 2022 at "ACS synthetic biology"

DOI: 10.1021/acssynbio.1c00366

Abstract: There is a growing interest in establishing the methylotrophic yeast Pichia pastoris as microbial cell factories for producing fuels, chemicals, and natural products, particularly with methanol as the feedstock. Although CRISPR (Clustered Regularly Interspaced Short… read more here.

Keywords: recombination; integration; homology arms; efficiency ... See more keywords
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Proteome and genome integration analysis of obesity

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Published in 2023 at "Chinese Medical Journal"

DOI: 10.1097/cm9.0000000000002644

Abstract: Abstract The prevalence of obesity has increased worldwide in recent decades. Genetic factors are now known to play a substantial role in the predisposition to obesity and may contribute up to 70% of the risk… read more here.

Keywords: proteome genome; integration analysis; analysis; obesity ... See more keywords
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A plasmid toolset for CRISPR‐mediated genome editing and CRISPRi gene regulation in Escherichia coli

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Published in 2021 at "Microbial Biotechnology"

DOI: 10.1111/1751-7915.13780

Abstract: CRISPR technologies have become standard laboratory tools for genetic manipulations across all kingdoms of life. Despite their origins in bacteria, the development of CRISPR tools for engineering bacteria has been slower than for eukaryotes; nevertheless,… read more here.

Keywords: gene regulation; genome editing; genome integration; crispr ... See more keywords
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High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration

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Published in 2023 at "Science Advances"

DOI: 10.1126/sciadv.ade1285

Abstract: Efficient genome engineering is critical to understand and use microbial functions. Despite recent development of tools such as CRISPR-Cas gene editing, efficient integration of exogenous DNA with well-characterized functions remains limited to model bacteria. Here,… read more here.

Keywords: integration; specific genome; high throughput; site specific ... See more keywords