Genome mining and homologous comparison strategy for digging exporters contributing self-resistance in natamycin-producing Streptomyces strains
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-10131-7
Abstract: As antibiotics are always toxic to the antibiotic-producing strains themselves, most Streptomyces strains have evolved several self-resistance mechanisms, among which the antibiotic efflux system is understood best and is commonly found. Among the efflux systems,… read more here.
Keywords: natamycin producing; producing streptomyces; streptomyces strains; genome mining ... See more keywords
Natural product discovery through microbial genome mining.
Sign Up to like & getrecommendations! Published in 2020 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2020.07.010
Abstract: The advent of the genomic era has opened up enormous possibilities for the discovery of new natural products. Also known as specialized metabolites, these compounds produced by bacteria, fungi, and plants have long been sought… read more here.
Keywords: product discovery; biology; natural product; genome mining ... See more keywords
Fungal feruloyl esterases: Functional validation of genome mining based enzyme discovery including uncharacterized subfamilies.
Sign Up to like & getrecommendations! Published in 2018 at "New biotechnology"
DOI: 10.1016/j.nbt.2017.11.004
Abstract: Feruloyl esterases (FAEs) are a diverse group of enzymes that specifically catalyze the hydrolysis of ester bonds between a hydroxycinnamic (e.g. ferulic) acid and plant poly- or oligosaccharides. FAEs as auxiliary enzymes significantly assist xylanolytic… read more here.
Keywords: feruloyl esterases; faes; fungal faes; genome mining ... See more keywords
Expanding the Chemical Diversity of Fasamycin Via Genome Mining and Biocatalysis.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of natural products"
DOI: 10.1021/acs.jnatprod.1c01089
Abstract: Genome mining and biocatalytic modification of chemical structures are critical methods to develop new antibiotics. In this study, eight new fasamycins (3, 4, 6, and 8-12) along with five known analogues (1, 2, 5, 7,… read more here.
Keywords: chemical diversity; genome mining;
Incarnatapeptins A and B, Nonribosomal Peptides Discovered Using Genome Mining and 1H/15N HSQC-TOCSY.
Sign Up to like & getrecommendations! Published in 2020 at "Organic letters"
DOI: 10.1021/acs.orglett.0c00818
Abstract: Methods for the focused isolation of low-abundance natural products with specific chemical substructures could expand known bioactive chemical diversity for drug discovery. Here we report the combined use of genome mining and an 15N NMR-based… read more here.
Keywords: nonribosomal peptides; incarnatapeptins nonribosomal; mining 15n; genome mining ... See more keywords
Griseocazines: Neuroprotective Multiprenylated Cyclodipeptides Identified through Targeted Genome Mining.
Sign Up to like & getrecommendations! Published in 2022 at "Organic letters"
DOI: 10.1021/acs.orglett.2c00745
Abstract: Prenylation can impart pharmacological advantages to bioactive compounds. Global genome mining for prenylated cyclodipeptides identified a gczABC BGC from Streptomyces griseocarneus 132 containing a cyclodipeptide synthase and two prenyltransferase genes. Subsequent heterologous expression allowed isolation… read more here.
Keywords: neuroprotective multiprenylated; griseocazines neuroprotective; cyclodipeptides identified; genome mining ... See more keywords
Discovery of a Cyclic Depsipeptide from Chaetomium mollipilium by the Genome Mining Approach.
Sign Up to like & getrecommendations! Published in 2022 at "Organic letters"
DOI: 10.1021/acs.orglett.2c01172
Abstract: Genome mining and bioinformatics analyses allowed us to rationally find a candidate biosynthetic gene cluster for a new cyclic depsipeptide of Chaetomium mollipilium. A heterologous reconstitution of the identified biosynthetic pathway predictably afforded a new… read more here.
Keywords: genome mining; cyclic depsipeptide; chaetomium mollipilium; depsipeptide ... See more keywords
Genome Mining and Metabolomics Unveil Pseudonochelin: A Siderophore Containing 5-Aminosalicylate from a Marine-Derived Pseudonocardia sp. Bacterium.
Sign Up to like & getrecommendations! Published in 2022 at "Organic letters"
DOI: 10.1021/acs.orglett.2c01408
Abstract: Pseudonochelin (1), a siderophore from a marine-derived Pseudonocardia sp. bacterium, was discovered using genome mining and metabolomics technologies. A 5-aminosalicylic acid (5-ASA) unit, not previously found in siderophore natural products, was identified in 1. Annotation… read more here.
Keywords: genome mining; pseudonocardia bacterium; mining metabolomics; marine derived ... See more keywords
Genome Mining and Activation of a Silent PKS/NRPS Gene Cluster Direct the Production of Totopotensamides.
Sign Up to like & getrecommendations! Published in 2017 at "Organic letters"
DOI: 10.1021/acs.orglett.7b02878
Abstract: A 92 kb silent hybrid polyketide and nonribosomal peptide gene cluster in marine-derived Streptomyces pactum SCSIO 02999 was activated by genetically manipulating the regulatory genes, including the knockout of two negative regulators (totR5 and totR3)… read more here.
Keywords: genome mining; direct production; gene cluster;
Genome Mining for Mycemycin: Discovery and Elucidation of Related Methylation and Chlorination Biosynthetic Chemistries.
Sign Up to like & getrecommendations! Published in 2018 at "Organic letters"
DOI: 10.1021/acs.orglett.8b03373
Abstract: A silent dibenzoxazepinone (DBP) biosynthetic gene cluster ( myc) was mutagenically activated in Streptomyces olivaceus SCSIO T05, enabling the discovery of mycemycin C (4) and three new analogues [mycemycins F-H (1-3)]. Gene disruption, complementation experiments,… read more here.
Keywords: mycemycin; discovery; mining mycemycin; genome mining ... See more keywords
Genome Mining Reveals the Phosphonoalamide Natural Products and a New Route in Phosphonic Acid Biosynthesis.
Sign Up to like & getrecommendations! Published in 2020 at "ACS chemical biology"
DOI: 10.1021/acschembio.0c00256
Abstract: Phosphonic acid natural products have potent inhibitory activities that have led to their application as antibiotics. Recent studies uncovered large collections of gene clusters encoding for unknown phosphonic acids across microbial genomes. However, our limited… read more here.
Keywords: natural products; acid biosynthesis; phosphonic acid; genome mining ... See more keywords